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Transcriptome Profiling of Nasonia vitripennis Testis Reveals Novel Transcripts Expressed from the Selfish B Chromosome, Paternal Sex Ratio

Akbari, Omar S. and Antoshechkin, Igor and Hay, Bruce A. and Ferree, Patrick M. (2013) Transcriptome Profiling of Nasonia vitripennis Testis Reveals Novel Transcripts Expressed from the Selfish B Chromosome, Paternal Sex Ratio. G3, 3 (9). pp. 1597-1605. ISSN 2160-1836. PMCID PMC3755920. doi:10.1534/g3.113.007583. https://resolver.caltech.edu/CaltechAUTHORS:20131003-150639655

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Abstract

A widespread phenomenon in nature is sex ratio distortion of arthropod populations caused by microbial and genetic parasites. Currently little is known about how these agents alter host developmental processes to favor one sex or the other. The paternal sex ratio (PSR) chromosome is a nonessential, paternally transmitted centric fragment that segregates in natural populations of the jewel wasp, Nasonia vitripennis. To persist, PSR is thought to modify the hereditary material of the developing sperm, with the result that all nuclear DNA other than the PSR chromosome is destroyed shortly after fertilization. This results in the conversion of a fertilized embryo—normally a female—into a male, thereby insuring transmission of the “selfish” PSR chromosome, and simultaneously leading to wasp populations that are male-biased. To begin to understand this system at the mechanistic level, we carried out transcriptional profiling of testis from WT and PSR-carrying males. We identified a number of transcripts that are differentially expressed between these conditions. We also discovered nine transcripts that are uniquely expressed from the PSR chromosome. Four of these PSR-specific transcripts encode putative proteins, whereas the others have very short open reading frames and no homology to known proteins, suggesting that they are long noncoding RNAs. We propose several different models for how these transcripts could facilitate PSR-dependent effects. Our analyses also revealed 15.71 MB of novel transcribed regions in the N. vitripennis genome, thus increasing the current annotation of total transcribed regions by 53.4%. Finally, we detected expression of multiple meiosis-related genes in the wasp testis, despite the lack of conventional meiosis in the male sex.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1534/g3.113.007583DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3755920/PubMed CentralArticle
ORCID:
AuthorORCID
Akbari, Omar S.0000-0002-6853-9884
Antoshechkin, Igor0000-0002-9934-3040
Ferree, Patrick M.0000-0002-4315-4363
Additional Information:© 2013 Akbari et al. Received May 24, 2013. Accepted July 16, 2013. Communicating editor: S. R. Hawley. Data from this article have been deposited with the EMBL/GenBank Data Libraries under SRA accession nos. WT: SRX325702 (WT) and SRX325704 (PSR); corresponding BioSamples: SRS462062 (WT); SRS462063 (PSR); BioProject: SRP027543.
Issue or Number:9
PubMed Central ID:PMC3755920
DOI:10.1534/g3.113.007583
Record Number:CaltechAUTHORS:20131003-150639655
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20131003-150639655
Official Citation:Transcriptome Profiling of Nasonia vitripennis Testis Reveals Novel Transcripts Expressed from the Selfish B Chromosome, Paternal Sex Ratio Omar S. Akbari, Igor Antoshechkin, Bruce A. Hay, and Patrick M. Ferree G3 September 2013 3:1597-1605; Early Online July 26, 2013, doi:10.1534/g3.113.007583
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:41663
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:04 Oct 2013 18:23
Last Modified:10 Nov 2021 04:32

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