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Designing libraries of chimeric proteins using SCHEMA recombination and RASPP

Smith, Matthew A. and Arnold, Frances H. (2014) Designing libraries of chimeric proteins using SCHEMA recombination and RASPP. In: Directed Evolution Library Creation: Methods and Protocols. Methods in Molecular Biology. No.1179. Humana Press , New York, NY, pp. 335-343. ISBN 978-1-4939-1052-6.

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SCHEMA is a method for designing libraries of novel proteins by recombination of homologous sequences. The goal is to maximize the number of folded proteins while simultaneously generating significant sequence diversity. Here, we use the RASPP algorithm to identify optimal SCHEMA designs for shuffling contiguous elements of sequence. To exemplify the method, SCHEMA is used to recombine five fungal cellobiohydrolases (CBH1s) to produce a library of more than 390,000 novel CBH1 sequences.

Item Type:Book Section
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URLURL TypeDescription Paper
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2014 Springer. The authors acknowledge funding from the Institute for Collaborative Biotechnologies through grant W911NF-09-D-0001 from the U.S. Army Research Office and The National Central University,Taiwan, through a Cooperative Agreement for Energy Research Collaboration. MAS is supported by a Resnick Sustainability Institute fellowship.
Group:Resnick Sustainability Institute
Funding AgencyGrant Number
Army Research Office (ARO)W911NF-09-D-001
National Central University (Taiwan)UNSPECIFIED
Resnick Sustainability InstituteUNSPECIFIED
Subject Keywords:Protein engineering Homologous recombination SCHEMA RASPP Chimeragenesis
Series Name:Methods in Molecular Biology
Issue or Number:1179
Record Number:CaltechAUTHORS:20131015-081653940
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:41910
Deposited By: Joy Painter
Deposited On:15 Oct 2013 20:28
Last Modified:10 Nov 2021 04:35

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