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Designing libraries of chimeric proteins using SCHEMA recombination and RASPP

Smith, Matthew A. and Arnold, Frances H. (2014) Designing libraries of chimeric proteins using SCHEMA recombination and RASPP. In: Directed Evolution Library Creation: Methods and Protocols. Methods in Molecular Biology. No.1179. Humana Press , New York, NY, pp. 335-343. ISBN 978-1-4939-1052-6. http://resolver.caltech.edu/CaltechAUTHORS:20131015-081653940

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Abstract

SCHEMA is a method for designing libraries of novel proteins by recombination of homologous sequences. The goal is to maximize the number of folded proteins while simultaneously generating significant sequence diversity. Here, we use the RASPP algorithm to identify optimal SCHEMA designs for shuffling contiguous elements of sequence. To exemplify the method, SCHEMA is used to recombine five fungal cellobiohydrolases (CBH1s) to produce a library of more than 390,000 novel CBH1 sequences.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://www.che.caltech.edu/groups/fha/media/SCHEMA_MIMB.pdfAuthorDiscussion Paper
http://dx.doi.org/10.1007/978-1-4939-1053-3_22DOIArticle
http://link.springer.com/protocol/10.1007%2F978-1-4939-1053-3_22PublisherArticle
ORCID:
AuthorORCID
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2014 Springer. The authors acknowledge funding from the Institute for Collaborative Biotechnologies through grant W911NF-09-D-0001 from the U.S. Army Research Office and The National Central University,Taiwan, through a Cooperative Agreement for Energy Research Collaboration. MAS is supported by a Resnick Sustainability Institute fellowship.
Group:Resnick Sustainability Institute
Funders:
Funding AgencyGrant Number
Army Research Office (ARO)W911NF-09-D-001
National Central University (Taiwan)UNSPECIFIED
Resnick Sustainability InstituteUNSPECIFIED
Subject Keywords:Protein engineering Homologous recombination SCHEMA RASPP Chimeragenesis
Record Number:CaltechAUTHORS:20131015-081653940
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20131015-081653940
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:41910
Collection:CaltechAUTHORS
Deposited By: Joy Painter
Deposited On:15 Oct 2013 20:28
Last Modified:08 Sep 2017 23:06

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