CaltechAUTHORS
  A Caltech Library Service

Structural Organization of the 19S Proteasome Lid: Insights from MS of Intact Complexes

Sharon, Michal and Taverner, Thomas and Ambroggio, Xavier I. and Deshaies, Raymond J. and Robinson, Carol V. (2006) Structural Organization of the 19S Proteasome Lid: Insights from MS of Intact Complexes. PLoS Biology, 4 (8). e267. ISSN 1544-9173. PMCID PMC1523230. https://resolver.caltech.edu/CaltechAUTHORS:SHAplosbio06

[img]
Preview
PDF - Published Version
Creative Commons Attribution.

2042Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:SHAplosbio06

Abstract

The 26S proteasome contains a 19S regulatory particle that selects and unfolds ubiquitinated substrates for degradation in the 20S catalytic particle. To date there are no high-resolution structures of the 19S assembly, nor of the lid or base subcomplexes that constitute the 19S. Mass spectra of the intact lid complex from Saccharomyces cerevisiae show that eight of the nine subunits are present stoichiometrically and that a stable tetrameric subcomplex forms in solution. Application of tandem mass spectrometry to the intact lid complex reveals the subunit architecture, while the coupling of a cross-linking approach identifies further interaction partners. Taking together our results with previous analyses we are able to construct a comprehensive interaction map. In summary, our findings allow us to identify a scaffold for the assembly of the particle and to propose a regulatory mechanism that prevents exposure of the active site until assembly is complete. More generally, the results highlight the potential of mass spectrometry to add crucial insight into the structural organization of an endogenous, wild-type complex.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1523230/PubMed CentralArticle
http://biology.plosjournals.org/perlserv/?request=get-document&doi=10.1371/journal.pbio.0040267OtherUNSPECIFIED
http://biology.plosjournals.org/perlserv/?request=get-document&doi=10.1371/journal.pbio.0040267OtherUNSPECIFIED
ORCID:
AuthorORCID
Deshaies, Raymond J.0000-0002-3671-9354
Additional Information:© 2006 Sharon et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received: March 31, 2006; Accepted: June 9, 2006; Published: August 1, 2006 M.S. is grateful for funding from the European Molecular Biology Organization and from the Wingate Scholarship. We thank Rati Verma and Sarah Maslen for valuable assistance in the preparation of this manuscript. CVR acknowledges the Walters-Kundert trust. Author contributions: MS, RJD, and CVR conceived and designed the experiments. MS and TT performed the experiments. MS and TT analyzed the data. XIA and RJD contributed reagents/materials/analysis tools. MS, RJD, and CVR wrote the paper. Competing interests: The authors have declared that no competing interests exist.
Funders:
Funding AgencyGrant Number
European Molecular Biology Organization (EMBO)UNSPECIFIED
Wingate ScholarshipUNSPECIFIED
Walters-Kundert trustUNSPECIFIED
Issue or Number:8
PubMed Central ID:PMC1523230
Record Number:CaltechAUTHORS:SHAplosbio06
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:SHAplosbio06
Official Citation:Sharon M, Taverner T, Ambroggio XI, Deshaies RJ, Robinson CV (2006) Structural Organization of the 19S Proteasome Lid: Insights from MS of Intact Complexes. PLoS Biol 4(8): e267
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4202
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:07 Aug 2006
Last Modified:02 Oct 2019 23:10

Repository Staff Only: item control page