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A detailed description of an economical setup for electroporation of chick embryos in ovo

Borges, R. M. and Horne, J. H. and Melo, A. and Vidal, J. T. and Vieceli, F. M. and Melo, M. O. and Kanno, T. Y. N. and Fraser, S. E. and Yan, C. Y. I. (2013) A detailed description of an economical setup for electroporation of chick embryos in ovo. Brazilian Journal of Medical and Biological Research, 46 (9). pp. 752-757. ISSN 0100-879X. PMCID PMC3854436. https://resolver.caltech.edu/CaltechAUTHORS:20131025-104350940

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Abstract

One of the challenges of the postgenomic era is characterizing the function and regulation of specific genes. For various reasons, the early chick embryo can easily be adopted as an in vivo assay of gene function and regulation. The embryos are robust, accessible, easily manipulated, and maintained in the laboratory. Genomic resources centered on vertebrate organisms increase daily. As a consequence of optimization of gene transfer protocols by electroporation, the chick embryo will probably become increasingly popular for reverse genetic analysis. The challenge of establishing chick embryonic electroporation might seem insurmountable to those who are unfamiliar with experimental embryological methods. To minimize the cost, time, and effort required to establish a chick electroporation assay method, we describe and illustrate in great detail the procedures involved in building a low-cost electroporation setup and the basic steps of electroporation.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-879X2013000900752&lng=en&nrm=iso/&tlng=enPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854436/PubMed CentralArticle
ORCID:
AuthorORCID
Vieceli, F. M.0000-0001-5142-8224
Fraser, S. E.0000-0002-5377-0223
Additional Information:© 2013 Associação Brasileira de Divulgação Científica. Received May 15, 2013. Accepted June 7, 2013. First published online September 16, 2013. The authors would like to thank Drs. Shankar Srinivas (Oxford University) and Cathy Krull (University of Michigan Medical School) for sharing the plasmids pCS2-TdTomato-2A-H2bGFP and pMES, respectively. Research partially supported by FAPESP and CNPq.
Funders:
Funding AgencyGrant Number
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNSPECIFIED
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNSPECIFIED
Subject Keywords:Electroporation; In vivo gene expression; Electroporator; Vertebrate embryo
Issue or Number:9
PubMed Central ID:PMC3854436
Record Number:CaltechAUTHORS:20131025-104350940
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20131025-104350940
Official Citation:BORGES, R.M. et al. A detailed description of an economical setup for electroporation of chick embryos in ovo. Braz J Med Biol Res [online]. 2013, vol.46, n.9, pp. 752-757. Epub Sep 18, 2013. ISSN 1414-431X. http://dx.doi.org/10.1590/1414-431X20133232.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:42068
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:29 Oct 2013 18:12
Last Modified:03 Oct 2019 05:54

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