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Innovation by homologous recombination

Trudeau, Devin L. and Smith, Matthew A. and Arnold, Frances H. (2013) Innovation by homologous recombination. Current Opinion in Chemical Biology, 17 (6). pp. 902-909. ISSN 1367-5931. https://resolver.caltech.edu/CaltechAUTHORS:20131030-110816201

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Abstract

Swapping fragments among protein homologs can produce chimeric proteins with a wide range of properties, including properties not exhibited by the parents. Computational methods that use information from structures and sequence alignments have been used to design highly functional chimeras and chimera libraries. Recombination has generated proteins with diverse thermostability and mechanical stability, enzyme substrate specificity, and optogenetic properties. Linear regression, Gaussian processes, and support vector machine learning have been used to model sequence-function relationships and predict useful chimeras. These approaches enable engineering of protein chimeras with desired functions, as well as elucidation of the structural basis for these functions.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.cbpa.2013.10.007DOIArticle
http://www.sciencedirect.com/science/article/pii/S1367593113001750PublisherArticle
ORCID:
AuthorORCID
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2013 Elsevier Ltd. Available online 29 October 2013. The authors thank Claire Bedbrook for helpful discussions. This work was supported by the Institute for Collaborative Biotechnologies through grant [W911NF-09-D-0001] from the U.S. Army Research and and the National Science Council of Taiwan, R.O.C., through its grant no. NSC 102-3113-P-008-001. DLT is supported by a Canadian National Science and Engineering Research Council post-graduate fellowship. MAS was supported by a Resnick Sustainability Institute fellowship.
Group:Resnick Sustainability Institute
Funders:
Funding AgencyGrant Number
Army Research Office (ARO)W911 NF-09-D-001
National Science Council (Taipei)NSC 102-3113-P-008-001
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
Resnick Sustainability InstituteUNSPECIFIED
Issue or Number:6
Record Number:CaltechAUTHORS:20131030-110816201
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20131030-110816201
Official Citation:Devin L Trudeau, Matthew A Smith, Frances H Arnold, Innovation by homologous recombination, Current Opinion in Chemical Biology, Volume 17, Issue 6, December 2013, Pages 902-909, ISSN 1367-5931, http://dx.doi.org/10.1016/j.cbpa.2013.10.007. (http://www.sciencedirect.com/science/article/pii/S1367593113001750)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:42138
Collection:CaltechAUTHORS
Deposited By: Heidi Rusina
Deposited On:30 Oct 2013 19:32
Last Modified:03 Oct 2019 05:55

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