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Methanobrevibacter filiformis sp. nov., a filamentous methanogen from termite hindguts

Leadbetter, Jared R. and Crosby, Laurel D. and Breznak, John A. (1998) Methanobrevibacter filiformis sp. nov., a filamentous methanogen from termite hindguts. Archives of Microbiology, 169 (4). pp. 287-292. ISSN 0302-8933. https://resolver.caltech.edu/CaltechAUTHORS:20131031-112409884

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Abstract

A morphologically distinct, filamentous methanogen was isolated from hindguts of the subterranean termite, Reticulitermes flavipes (Kollar) (Rhinotermitidae), wherein it was part of the microbiota colonizing the hindgut wall. Individual filaments of strain RFM-3 were 0.23–0.28 μm in diameter and usually > 50 μm in length and aggregated into flocs that were often ≥ 0.1 mm in diameter. Optimal growth of strain RFM-3 was obtained at pH 7.0–7.2 and 30° C with a yeast-extract-supplemented, dithiothreitol-reduced medium in which cells produced stoichiometric amounts of methane from H_2 + CO_2. The morphology and gram-positive staining reaction of strain RFM-3, as well as its resistance to cell lysis by various chemical agents and its restriction to H_2 + CO_2 as an energy source, suggested that it was a member of the Methanobacteriaceae. The nucleotide sequence of the SSU-rRNA-encoding gene of strain RFM-3 confirmed this affiliation and also supported its recognition as a new species of Methanobrevibacter, for which the epithet filiformis is herewith proposed. Although M. filiformis was one of the dominant methanogens in R. flavipes collected from Woods Hole (Mass., USA), cells of similar morphology were not consistently observed in R. flavipes collected from different geographical locations.


Item Type:Article
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http://dx.doi.org/10.1007/s002030050574 DOIArticle
https://rdcu.be/bzXz7PublisherFree ReadCube access
ORCID:
AuthorORCID
Leadbetter, Jared R.0000-0002-7033-0844
Additional Information:© 1998 Springer-Verlag. Received: 15 September 1997; Accepted: 20 November 1997. This research was funded by National Science Foundation grant nos. IBN91-06636 (to J.A. Breznak) and BIR91-20006 (to the MSU Center for Microbial Ecology). Electron microscopy was performed by H.S. Pankratz, to whom we are very grateful. We also thank D. Stahl for kindly providing the unpublished SSU rRNA sequence of M. smithii, a result of work supported by NSF grant no. DEB-9408243.
Funders:
Funding AgencyGrant Number
NSFIBN91-06636
NSFBIR91-20006
NSFDEB-9408243
Subject Keywords:archaea; Methanobacteriaceae; gut microbiota; symbiosis; insect
Issue or Number:4
Record Number:CaltechAUTHORS:20131031-112409884
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20131031-112409884
Official Citation:Leadbetter, J., Crosby, L. & Breznak, J. Arch Microbiol (1998) 169: 287. https://doi.org/10.1007/s002030050574
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:42156
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:31 Oct 2013 21:42
Last Modified:03 Oct 2019 05:55

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