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The Sixteenth Iron in the Nitrogenase MoFe Protein

Zhang, Limei and Kaiser, Jens T. and Meloni, Gabriele and Yang, Kun-Yun and Spatzal, Thomas and Andrade, Susana L. A. and Einsle, Oliver and Howard, James B. and Rees, Douglas C. (2013) The Sixteenth Iron in the Nitrogenase MoFe Protein. Angewandte Chemie International Edition, 52 (40). pp. 10529-10532. ISSN 1433-7851. PMCID PMC3891402.

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Another iron in the fire: X-ray anomalous diffraction studies on the nitrogenase MoFe protein show the presence of a mononuclear iron site, designated as Fe16, which was previously identified as either Ca^(2+) or Mg^(2+). The position of the absorption edge indicates that this site is in the oxidation state +2. The high sequence conservation of the residues coordinated to Fe16 emphasizes the potential importance of the site in nitrogenase.

Item Type:Article
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URLURL TypeDescription CentralArticle
Kaiser, Jens T.0000-0002-5948-5212
Spatzal, Thomas0000-0002-9136-5915
Rees, Douglas C.0000-0003-4073-1185
Additional Information:© 2013 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Received: May 6, 2013. Published online: August 21, 2013. The work was supported by the NIH (grant GM45162 to D.C.R.), NSERC PDF award (to L.M.Z.), and Deutsche Forschungsgemeinschaft (grants Ei-520/7 to O.E., An-676/1 to S.L.A.A., and IRTG 1478). G.M. is a Marie Curie International Outgoing Fellow (European Commission, grant no. 252961). We thank staff at Beamline 12–2, Stanford Synchrotron Radiation Lightsource (SSRL) and staff at HXMA Beamline, Canada Light Source (CLS). SSRL is operated for the DOE and supported by its OBER and by the NIH, NIGMS (P41GM103393) and the NCRR (P41RR001209). CLS is supported by NSERC, NRC, CIHR, and the University of Saskatchewan. We acknowledge the Gordon and Betty Moore Foundation, the Beckman Institute, and the Sanofi-Aventis Bioengineering Research Program at Caltech for their generous support of the Molecular Observatory at Caltech. The assistance of Nathan Dalleska and the resources of the Caltech Environmental Analysis Center are greatly appreciated.
Funding AgencyGrant Number
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
Deutsche Forschungsgemeinschaft (DFG)Ei-520/7
Deutsche Forschungsgemeinschaft (DFG)An-676/1
Deutsche Forschungsgemeinschaft (DFG)IRTG 1478
Marie Curie Fellowship252961
National Research Council of CanadaUNSPECIFIED
Canadian Institutes of Health Research (CIHR)UNSPECIFIED
University of SaskatchewanUNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
Caltech Sanofi-Aventis Bioengineering Research ProgramUNSPECIFIED
Subject Keywords:iron; metalloproteins; multiple-wavelength anomalous diffraction; nitrogen fixation; nitrogenases
Issue or Number:40
PubMed Central ID:PMC3891402
Record Number:CaltechAUTHORS:20131104-141900919
Persistent URL:
Official Citation:Zhang, L., Kaiser, J. T., Meloni, G., Yang, K.-Y., Spatzal, T., Andrade, S. L. A., Einsle, O., Howard, J. B. and Rees, D. C. (2013), The Sixteenth Iron in the Nitrogenase MoFe Protein . Angew. Chem. Int. Ed., 52: 10529–10532. doi: 10.1002/anie.201303877
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:42229
Deposited By: Ruth Sustaita
Deposited On:04 Nov 2013 22:44
Last Modified:09 Mar 2020 13:18

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