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Fast Metabolic Response to Drug Intervention Through Analysis on a Miniaturized, Highly Integrated Molecular Imaging System

Wang, Jun and Hwang, Kiwook and Braas, Daniel and Dooraghi, Alex and Nathanson, David and Campbell, Dean O. and Gu, Yuchao and Sandberg, Troy and Mischel, Paul and Radu, Caius and Chatziioannou, Arion F. and Phelps, Michael E. and Christofk, Heather and Heath, James R. (2013) Fast Metabolic Response to Drug Intervention Through Analysis on a Miniaturized, Highly Integrated Molecular Imaging System. Journal of Nuclear Medicine, 54 (10). pp. 1820-1824. ISSN 0161-5505 . PMCID PMC4106462. https://resolver.caltech.edu/CaltechAUTHORS:20131105-095711532

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Abstract

We report on a radiopharmaceutical imaging platform designed to capture the kinetics of cellular responses to drugs. Methods: A portable in vitro molecular imaging system comprising a microchip and a β-particle imaging camera permitted routine cell-based radioassays of small numbers of either suspended or adherent cells. We investigated the kinetics of responses of model lymphoma and glioblastoma cancer cell lines to ^(18)F-FDG uptake after drug exposure. Those responses were correlated with kinetic changes in the cell cycle or with changes in receptor tyrosine kinase signaling. Results: The platform enabled direct radioassays of multiple cell types and yielded results comparable to those from conventional approaches; however, the platform used smaller sample sizes, permitted a higher level of quantitation, and did not require cell lysis. Conclusion: The kinetic analysis enabled by the platform provided a rapid (∼1 h) drug screening assay.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.2967/jnumed.112.118497DOIArticle
http://jnm.snmjournals.org/content/54/10/1820PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4106462/PubMed CentralArticle
ORCID:
AuthorORCID
Heath, James R.0000-0001-5356-4385
Additional Information:© 2013 Society of Nuclear Medicine and Molecular Imaging, Inc. Received Dec. 20, 2012; revision accepted May 14, 2013. Published online Aug. 26, 2013. The costs of publication of this article were defrayed in part by the payment of page charges. Therefore, and solely to indicate this fact, this article is hereby marked “advertisement” in accordance with 18 USC section 1734. This work was funded by the National Cancer Institute (5U54 CA119347, to James R. Heath), the National Institute of Neurological Disorders and Stroke (NS 73831, to Paul Mischel), the Ben and Catherine Ivy Foundation, and the Department of Energy (DE-SC0001249). Kiwook Hwang acknowledges the Samsung Foundation for a fellowship. Some of the authors (Caius Radu, Arion F. Chatziioannou, Michael E. Phelps, and James R. Heath) have ownership in Sofie Biosciences, which has rights to the ^(18)F-FAC probe. No other potential conflict of interest relevant to this article was reported. We thank David Baltimore for access to the Nikon Diaphot 200 fluorescence microscope.
Funders:
Funding AgencyGrant Number
NIH5U54 CA119347
NIHNS 73831
Ben and Catherine Ivy FoundationUNSPECIFIED
Department of Energy (DOE)DE-SC0001249
Samsung FoundationUNSPECIFIED
National Cancer InstituteUNSPECIFIED
National Institute of Neurological Disorders and Stroke (NINDS)UNSPECIFIED
Subject Keywords:microfluidics; molecular imaging; radiopharmaceuticals radioassay
Issue or Number:10
PubMed Central ID:PMC4106462
Record Number:CaltechAUTHORS:20131105-095711532
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20131105-095711532
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:42250
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:05 Nov 2013 23:48
Last Modified:03 Oct 2019 05:56

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