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Recent advances in electron cryotomography and their application to imaging purified protein complexes, isolated organelles, and small cells

Iancu, Cristina V. and Murphy, Gavin E. and Wright, Elizabeth R. and Benjamin, Jordan and Tivol, William F. and Dias, D. Prabha and Heymann, J. Bernard and Jensen, Grant (2005) Recent advances in electron cryotomography and their application to imaging purified protein complexes, isolated organelles, and small cells. Microscopy and Microanalysis, 11 (S2). pp. 1052-1053. ISSN 1431-9276. doi:10.1017/S1431927605509255. https://resolver.caltech.edu/CaltechAUTHORS:20131112-082151375

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Abstract

A new 300kV, FEG, helium-cooled, energy-filtered "G2 Polara" electron cryoTEM was recently installed at Caltech. Using this instrument, we have now extensively tested the potential advantages of liquid helium cooling and the inclusion of two orthogonal tilt-series in the context of electron cryotomography. These results and the application of the technology to several specific biological problems are reported.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1017/S1431927605509255DOIArticle
http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=327339PublisherArticle
ORCID:
AuthorORCID
Iancu, Cristina V.0000-0002-7352-9226
Jensen, Grant0000-0003-1556-4864
Additional Information:© 2005 Microscopy Society of America. Published online: 01 August 2005. Extended abstract of a paper presented at Microscopy and Microanalysis 2005 in Honolulu, Hawaii, USA, July 31-August 4, 2005.
Issue or Number:S2
DOI:10.1017/S1431927605509255
Record Number:CaltechAUTHORS:20131112-082151375
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20131112-082151375
Official Citation:C V Iancu, G E Murphy, E R Wright, J Benjamin, W F Tivol, D P Dias, J B Heymann and G Jensen (2005). Recent Advances in Electron Cryotomography and their Application to Imaging Purified Protein Complexes, Isolated Organelles, and Small Cells. Microscopy and Microanalysis, 11 (Suppl. 02) , pp 1052-1053. doi:10.1017/S1431927605509255.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:42365
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:12 Nov 2013 23:27
Last Modified:10 Nov 2021 16:22

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