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Oxidation of Arsenite by Agrobacterium albertimagni, AOL15, sp. nov., Isolated from Hot Creek, California

Salmassi, Tina M. and Venkateswaren, Kasthuri and Satomi, Masataka and Nealson, Kenneth H. and Newman, Dianne K. and Hering, Janet G. (2002) Oxidation of Arsenite by Agrobacterium albertimagni, AOL15, sp. nov., Isolated from Hot Creek, California. Geomicrobiology Journal, 19 (1). pp. 53-66. ISSN 0149-0451. http://resolver.caltech.edu/CaltechAUTHORS:20131127-130752075

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Abstract

An arsenite-oxidizing bacterium, Agrobacterium albertimagni strain AOL15 (ATCC BAA-24), was isolated from the surface of aquatic macrophytes collected in Hot Creek, California. Under laboratory conditions, whole cell suspensions of AOL15 oxidized arsenite with a K_s of 3.4 ± 2.2µM and a V_(Max) of 1.81 ± 0.58 x 10^(-12) µmole · cell^(-1)· min^(-1) (or 0.043 ± 0.017 µmole · mg protein^(-1)· min^(-1)). The K_s value for AOL15 is the lowest value to date reported for whole cell suspensions and is comparable to ambient concentrations of arsenic of 2.7 µM reported for Hot Creek, indicating that AOL15 can oxidize arsenite under ambient conditions. Previous studies at this site revealed a rapid in situ oxidation of geothermally-derived arsenite while field incubation studies demonstrated that this oxidation was bacterially mediated. The isolation of the arsenite oxidizer AOL15 from this environment supports these previous observations. Arsenite does not support chemolithoautotrophic growth of AOL15 and toxicity studies with AOL15 showed that arsenite (at 5 mM) is toxic to AOL15, yet arsenate concentrations as high as 50 mM do not show any toxic effects. These results suggest that the oxidation of arsenite by AOL15 is a detoxification mechanism.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1080/014904502317246165DOIArticle
http://www.tandfonline.com/doi/abs/10.1080/014904502317246165PublisherArticle
ORCID:
AuthorORCID
Nealson, Kenneth H.0000-0001-5189-3732
Newman, Dianne K.0000-0003-1647-1918
Additional Information:© 2002 Taylor & Francis. Received 22 March 2001; accepted 3 July 2001. Published online: 10 Nov 2010. The authors would like to acknowledge Dr. Steve Lindow (University of California Davis) for the identification of the macrophytes , Dr. Brian Lanoil (Caltech) for assistance with the phylogenetic analysis, Bridget L.West (Caltech) for assistance with the kinetics experiments, and Prof. Jared Leadbetter (Caltech) for helpful discussion. This work was funded in part by the National Science Foundation (EAR-9807662).
Funders:
Funding AgencyGrant Number
NSFEAR-9807662
Subject Keywords:arsenite-oxidation, Agrobacterium albertimagni strain AOL15, arsenite, arsenic, redox, microbial oxidation
Record Number:CaltechAUTHORS:20131127-130752075
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20131127-130752075
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:42757
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:02 Dec 2013 16:31
Last Modified:23 Oct 2017 21:48

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