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Luminescence of [Ru(bpy)_2(dppz)]^(2+) Bound to RNA Mismatches

McConnell, Anna J. and Song, Hang and Barton, Jacqueline K. (2013) Luminescence of [Ru(bpy)_2(dppz)]^(2+) Bound to RNA Mismatches. Inorganic Chemistry, 52 (17). pp. 10131-10136. ISSN 0020-1669. PMCID PMC3823503. doi:10.1021/ic401531r. https://resolver.caltech.edu/CaltechAUTHORS:20131204-111813799

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Abstract

The luminescence of rac-[Ru(bpy)_2(dppz)]^(2+) (bpy = 2,2′-bipyridine and dppz = dipyrido[3,2-a:2′,3′-c]phenazine) was explored in the presence of RNA oligonucleotides containing a single RNA mismatch (CA and GG) in order to develop a probe for RNA mismatches. While there is minimal luminescence of [Ru(bpy)_2(dppz)]^(2+) in the presence of matched RNA due to weak binding, the luminescence is significantly enhanced in the presence of a single CA mismatch. The luminescence differential between CA mismatched and matched RNA is substantially higher compared to the DNA analogue, and therefore, [Ru(bpy)_2(dppz)]^(2+) appears to be also a sensitive light switch probe for a CA mismatch in duplex RNA. Although the luminescence intensity is lower in the presence of RNA than DNA, Förster resonance energy transfer (FRET) between the donor ruthenium complex and FRET acceptor SYTO 61 is successfully exploited to amplify the luminescence in the presence of the mismatch. Luminescence and quenching studies with sodium iodide suggest that [Ru(bpy)_2(dppz)]^(2+) binds to these mismatches via metalloinsertion from the minor groove. This work provides further evidence that metalloinsertion is a general binding mode of octahedral metal complexes to thermodynamically destabilized mismatches not only in DNA but also in RNA.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ic401531rDOIArticle
http://pubs.acs.org/doi/abs/10.1021/ic401531rPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823503/PubMed CentralArticle
ORCID:
AuthorORCID
McConnell, Anna J.0000-0001-7329-4319
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2013 American Chemical Society. Received: June 17, 2013; Published: August 22, 2013. We thank the NIH (GM33309 to J.K.B.) for their financial support, the Lindemann Trust for a postdoctoral fellowship to A.J.M., and the Tobacco-Related Disease Research Program (TRDRP) for a Dissertation Research Award to H.S. We are grateful to the Beckman Institute Laser Resource Center (BILRC) at Caltech for facilities.
Funders:
Funding AgencyGrant Number
NIHGM33309
Lindemann TrustUNSPECIFIED
California Tobacco-Related Disease Research ProgramUNSPECIFIED
Issue or Number:17
PubMed Central ID:PMC3823503
DOI:10.1021/ic401531r
Record Number:CaltechAUTHORS:20131204-111813799
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20131204-111813799
Official Citation:Luminescence of [Ru(bpy)2(dppz)]2+ Bound to RNA Mismatches Anna J. McConnell, Hang Song, and Jacqueline K. Barton Inorganic Chemistry 2013 52 (17), 10131-10136
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:42827
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:04 Dec 2013 21:42
Last Modified:10 Nov 2021 16:29

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