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DNA Protection by the Bacterial Ferritin Dps via DNA Charge Transport

Arnold, Anna R. and Barton, Jacqueline K. (2013) DNA Protection by the Bacterial Ferritin Dps via DNA Charge Transport. Journal of the American Chemical Society, 135 (42). pp. 15726-15729. ISSN 0002-7863. PMCID PMC3899832. doi:10.1021/ja408760w. https://resolver.caltech.edu/CaltechAUTHORS:20131206-091447174

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Abstract

Dps proteins, bacterial mini-ferritins that protect DNA from oxidative stress, are implicated in the survival and virulence of pathogenic bacteria. Here we examine the mechanism of E. coli Dps protection of DNA, specifically whether this DNA-binding protein can utilize DNA charge transport through the base pair π-stack to protect the genome from a distance. An intercalating ruthenium photooxidant was employed to generate DNA damage localized to guanine repeats, the sites of lowest potential in DNA. We find that Dps loaded with ferrous iron, in contrast to Apo-Dps and ferric iron-loaded Dps, significantly attenuates the yield of oxidative DNA damage. These data demonstrate that ferrous iron-loaded Dps is selectively oxidized to fill guanine radical holes, thereby restoring the integrity of the DNA. Luminescence studies indicate no direct interaction between the ruthenium photooxidant and Dps, supporting the DNA-mediated oxidation of ferrous iron-loaded Dps. Thus DNA charge transport may be a mechanism by which Dps efficiently protects the genome of pathogenic bacteria from a distance.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://pubs.acs.org/doi/abs/10.1021/ja408760wPublisherArticle
http://dx.doi.org/10.1021/ja408760wDOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3899832/PubMed CentralArticle
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2013 American Chemical Society. Received: August 23, 2013. Publication Date (Web): October 11, 2013. We thank Dr. Roberto Kolter at Harvard Medical School for his generous donation of the Dps plasmid and strain used for this study. We acknowledge the NIH for funding (GM49216). A.R.A. was supported by the National Institute on Aging of the NIH on a predoctoral NRSA (F31AG040954).
Funders:
Funding AgencyGrant Number
NIHGM49216
NIH Predoctoral FellowshipF31AG040954
National Institute on AgingUNSPECIFIED
Issue or Number:42
PubMed Central ID:PMC3899832
DOI:10.1021/ja408760w
Record Number:CaltechAUTHORS:20131206-091447174
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20131206-091447174
Official Citation:DNA Protection by the Bacterial Ferritin Dps via DNA Charge Transport Anna R. Arnold and Jacqueline K. Barton Journal of the American Chemical Society 2013 135 (42), 15726-15729
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:42877
Collection:CaltechAUTHORS
Deposited By:INVALID USER
Deposited On:06 Dec 2013 17:49
Last Modified:10 Nov 2021 16:29

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