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Functional Interrogation of Adult Hypothalamic Neurogenesis with Focal Radiological Inhibition

Lee, Daniel A. and Salvatierra, Juan and Velarde, Esteban and Wong, John and Ford, Eric C. and Blackshaw, Seth (2013) Functional Interrogation of Adult Hypothalamic Neurogenesis with Focal Radiological Inhibition. Journal of Visualized Experiments - Applied Physics, 81 . e50716. ISSN 1940-087X. https://resolver.caltech.edu/CaltechAUTHORS:20131209-132607211

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Abstract

The functional characterization of adult-born neurons remains a significant challenge. Approaches to inhibit adult neurogenesis via invasive viral delivery or transgenic animals have potential confounds that make interpretation of results from these studies difficult. New radiological tools are emerging, however, that allow one to noninvasively investigate the function of select groups of adult-born neurons through accurate and precise anatomical targeting in small animals. Focal ionizing radiation inhibits the birth and differentiation of new neurons, and allows targeting of specific neural progenitor regions. In order to illuminate the potential functional role that adult hypothalamic neurogenesis plays in the regulation of physiological processes, we developed a noninvasive focal irradiation technique to selectively inhibit the birth of adult-born neurons in the hypothalamic median eminence. We describe a method for Computer tomography-guided focal irradiation (CFIR) delivery to enable precise and accurate anatomical targeting in small animals. CFIR uses three-dimensional volumetric image guidance for localization and targeting of the radiation dose, minimizes radiation exposure to nontargeted brain regions, and allows for conformal dose distribution with sharp beam boundaries. This protocol allows one to ask questions regarding the function of adult-born neurons, but also opens areas to questions in areas of radiobiology, tumor biology, and immunology. These radiological tools will facilitate the translation of discoveries at the bench to the bedside.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.3791/50716DOIArticle
http://www.jove.com/video/50716PublisherArticle
ORCID:
AuthorORCID
Lee, Daniel A.0000-0001-7411-2740
Additional Information:© 2013 Journal of Visualized Experiments. Date published: 11/14/2013. We thank C. Montojo, J. Reyes, and M. Armour for technical advice and assistance. This work was supported by US National Institutes of Health grant F31 NS063550 (to D.A.L.), a Basil O'Connor Starter Scholar Award and grants from the Klingenstein Fund and NARSAD (to S.B.). S.B. is a W.M. Keck Distinguished Young Scholar in Medical Research. J.W. has a research funding and consultation agreements with Xstrahl, Inc.
Funders:
Funding AgencyGrant Number
NIHF31 NS063550
Klingenstein FundUNSPECIFIED
NARSADUNSPECIFIED
Xstrahl, IncUNSPECIFIED
Basil O’Connor Starter Scholar AwardUNSPECIFIED
W. M. Keck FoundationUNSPECIFIED
Subject Keywords:Neuroscience, Issue 81, Neural Stem Cells (NSCs), Body Weight, Radiotherapy, Image-Guided, Metabolism, Energy Metabolism, Neurogenesis, Cell Proliferation, Neurosciences, Irradiation, Radiological treatment, Computer-tomography (CT) imaging, Hypothalamus, Hypothalamic Proliferative Zone (HPZ), Median Eminence (ME), Small Animal Radiation Research Platform (SARRP)
Record Number:CaltechAUTHORS:20131209-132607211
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20131209-132607211
Official Citation:Lee, D.A., Salvatierra, J., Velarde, E., Wong, J., Ford, E.C., Blackshaw, S. Functional Interrogation of Adult Hypothalamic Neurogenesis with Focal Radiological Inhibition. J. Vis. Exp. (81), e50716, doi:10.3791/50716 (2013).
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:42906
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:12 Dec 2013 20:33
Last Modified:09 Mar 2020 13:18

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