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Biphasic influence of Miz1 on neural crest development by regulating cell survival and apical adhesion complex formation in the developing neural tube

Kerosuo, Laura and Bronner, Marianne E. (2014) Biphasic influence of Miz1 on neural crest development by regulating cell survival and apical adhesion complex formation in the developing neural tube. Molecular Biology of the Cell, 25 (3). pp. 347-355. ISSN 1059-1524. PMCID PMC3907275. http://resolver.caltech.edu/CaltechAUTHORS:20131209-133718257

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Abstract

Myc interacting zinc finger protein-1 (Miz1) is a transcription factor known to regulate cell cycle– and cell adhesion–related genes in cancer. Here we show that Miz1 also plays a critical role in neural crest development. In the chick, Miz1 is expressed throughout the neural plate and closing neural tube. Its morpholino-mediated knockdown affects neural crest precursor survival, leading to reduction of neural plate border and neural crest specifier genes Msx-1, Pax7, FoxD3, and Sox10. Of interest, Miz1 loss also causes marked reduction of adhesion molecules (N-cadherin, cadherin6B, and α1-catenin) with a concomitant increase of E-cadherin in the neural folds, likely leading to delayed and decreased neural crest emigration. Conversely, Miz1 overexpression results in up-regulation of cadherin6B and FoxD3 expression in the neural folds/neural tube, leading to premature neural crest emigration and increased number of migratory crest cells. Although Miz1 loss effects cell survival and proliferation throughout the neural plate, the neural progenitor marker Sox2 was unaffected, suggesting a neural crest–selective effect. The results suggest that Miz1 is important not only for survival of neural crest precursors, but also for maintenance of integrity of the neural folds and tube, via correct formation of the apical adhesion complex therein.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907275PubMed CentralArticle
http://www.molbiolcell.org/content/25/3/347.abstractPublisherArticle
http://dx.doi.org/10.1091/mbc.E13-06-0327DOIArticle
ORCID:
AuthorORCID
Kerosuo, Laura0000-0001-6710-3512
Bronner, Marianne E.0000-0003-4274-1862
Additional Information:© 2014 Kerosuo and Bronner. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). This article was published online ahead of print in MBoC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E13-06-0327) Received: Jun 19, 2013. Revised: Nov 7, 2013. Accepted: Nov 27, 2013. Published online before print December 4, 2013. This work was supported by the Sigrid Juselius Foundation, the Foundations’ Post doc Pool/Finnish Cultural Foundation, and the Ella and Georg Ehrnrooth Foundation (L.K.) and by National Institutes of Health Grant HD037105 to M.E.B.
Funders:
Funding AgencyGrant Number
Sigrid Juselius FoundationUNSPECIFIED
Finnish Cultural FoundationUNSPECIFIED
Ella och Georg Ehrnrooth’s FoundationUNSPECIFIED
NIHHD037105
PubMed Central ID:PMC3907275
Record Number:CaltechAUTHORS:20131209-133718257
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20131209-133718257
Official Citation:Laura Kerosuo and Marianne E. Bronner Biphasic influence of Miz1 on neural crest development by regulating cell survival and apical adhesion complex formation in the developing neural tube Mol. Biol. Cell 2014 25:3 347-355; First Published on December 4, 2013; doi:10.1091/mbc.E13-06-0327
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:42907
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:09 Dec 2013 22:02
Last Modified:25 Mar 2019 18:40

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