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Hemoglobin Synthesis in Rabbit Reticulocytes In vitro

Kruh, Jacques and Borsook, Henry (1956) Hemoglobin Synthesis in Rabbit Reticulocytes In vitro. Journal of Biological Chemistry, 220 (2). pp. 905-915. ISSN 0021-9258.

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We have reported the rapid incorporation of labeled amino acids into the proteins of rabbit reticulocytes in vitro and the stimulation of this process by certain amino acids, iron, fructose-amino acids, glucose, and some, as yet, unidentified material in the filtrate of boiled plasma. The incorporation was measured in the mixture of the total proteins of the reticulocytes (1, 2). In the study reported here two protein fractions were isolated, the hemoglobin, which comprises more than 75 per cent of the total, and the water-insoluble proteins. The hemoglobin was fractionated into heme and globin. Experiments were carried out with four C^(14)-labeled amino acids and with different stimulators to ascertain whether heme synthesis and amino acid incorporation went parallel or not.

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Additional Information:© 1955 American Society for Biochemistry and Molecular Biology. Received for publication, September 19, 1955. This study was aided by a contract between the Atomic Energy Commission and the Division of Biology, California Institute of Technology. It was also supported by a research grant from the National Institutes of Health, United States Public Health Service, and by a grant-in-aid from the American Cancer Society upon recommendation of the Committee on Growth of the National Research Council.
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Atomic Energy CommissionUNSPECIFIED
United States Public Health Service (USPHS)UNSPECIFIED
American Cancer SocietyUNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20131211-111009191
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ID Code:42956
Deposited By: Tony Diaz
Deposited On:11 Dec 2013 20:47
Last Modified:03 Oct 2019 06:03

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