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4D Cryo-Electron Microscopy of Proteins

Fitzpatrick, Anthony W. P. and Lorenz, Ulrich J. and Vanacore, Giovanni M. and Zewail, Ahmed H. (2013) 4D Cryo-Electron Microscopy of Proteins. Journal of the American Chemical Society, 131 (51). pp. 19123-19126. ISSN 0002-7863. doi:10.1021/ja4115055. https://resolver.caltech.edu/CaltechAUTHORS:20131216-084056795

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Abstract

Cryo-electron microscopy is a form of transmission electron microscopy that has been used to determine the 3D structure of biological specimens in the hydrated state and with high resolution. We report the development of 4D cryo-electron microscopy by integrating the fourth dimension, time, into this powerful technique. From time-resolved diffraction of amyloid fibrils in a thin layer of vitrified water at cryogenic temperatures, we were able to detect picometer movements of protein molecules on a nanosecond time scale. Potential future applications of 4D cryo-electron microscopy are numerous, and some are discussed here.


Item Type:Article
Related URLs:
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http://dx.doi.org/10.1021/ja4115055DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja4115055PublisherArticle
Additional Information:© 2013 American Chemical Society. Received: November 11, 2013; Publication Date (Web): December 6, 2013. The authors declare no competing financial interest. This work was supported by the National Science Foundation and the Air Force Office of Scientific Research in the Physical Biology Center for Ultrafast Science and Technology (UST) supported by the Gordon and Betty Moore Foundation at Caltech. A.W.P.F. is supported by a Marie Curie International Outgoing Fellowship. We thank A. W. McDowall and the Beckman Institute for help with sample preparations and for useful discussions.
Funders:
Funding AgencyGrant Number
NSFUNSPECIFIED
Air Force Office of Scientific Research (AFOSR)UNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Marie Curie International Outgoing FellowshipUNSPECIFIED
Issue or Number:51
DOI:10.1021/ja4115055
Record Number:CaltechAUTHORS:20131216-084056795
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20131216-084056795
Official Citation:Fitzpatrick, A. W. P., Lorenz, U. J., Vanacore, G. M., & Zewail, A. H. (2013). 4D Cryo-Electron Microscopy of Proteins. Journal of the American Chemical Society, 135(51), 19123-19126. doi: 10.1021/ja4115055
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:43012
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 Dec 2013 19:35
Last Modified:10 Nov 2021 16:31

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