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DNA-Modified Electrodes Fabricated Using Copper-Free Click Chemistry for Enhanced Protein Detection

Furst, Ariel L. and Hill, Michael G. and Barton, Jacqueline K. (2013) DNA-Modified Electrodes Fabricated Using Copper-Free Click Chemistry for Enhanced Protein Detection. Langmuir, 29 (52). pp. 16141-16149. ISSN 0743-7463. PMCID PMC3947573. doi:10.1021/la403262v.

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A method of DNA monolayer formation has been developed using copper-free click chemistry that yields enhanced surface homogeneity and enables variation in the amount of DNA assembled; extremely low-density DNA monolayers, with as little as 5% of the monolayer being DNA, have been formed. These DNA-modified electrodes (DMEs) were characterized visually, with AFM, and electrochemically, and were found to facilitate DNA-mediated reduction of a distally bound redox probe. These low-density monolayers were found to be more homogeneous than traditional thiol-modified DNA monolayers, with greater helix accessibility through an increased surface area-to-volume ratio. Protein binding efficiency of the transcriptional activator TATA-binding protein (TBP) was also investigated on these surfaces and compared to that on DNA monolayers formed with standard thiol-modified DNA. Our low-density monolayers were found to be extremely sensitive to TBP binding, with a signal decrease in excess of 75% for 150 nM protein. This protein was detectable at 4 nM, on the order of its dissociation constant, with our low-density monolayers. The improved DNA helix accessibility and sensitivity of our low-density DNA monolayers to TBP binding reflects the general utility of this method of DNA monolayer formation for DNA-based electrochemical sensor development.

Item Type:Article
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URLURL TypeDescription CentralArticle
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2013 American Chemical Society. Received: September 3, 2013; Revised: October 30, 2013. Publication Date (Web): December 11, 2013. We are grateful to the Office of Naval Research (N0014-09-1-1117) and the NIH (GM61077) for their financial support. We also thank Prof. Eileen Spain of Occidental College for input and helpful discussion for the AFM studies.
Funding AgencyGrant Number
Office of Naval Research (ONR)N0014-09-1-1117
Issue or Number:52
PubMed Central ID:PMC3947573
Record Number:CaltechAUTHORS:20131224-071741505
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Official Citation:DNA-Modified Electrodes Fabricated Using Copper-Free Click Chemistry for Enhanced Protein Detection Ariel L. Furst, Michael G. Hill, and Jacqueline K. Barton Langmuir 2013 29 (52), 16141-16149
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:43155
Deposited By: Tony Diaz
Deposited On:24 Dec 2013 17:41
Last Modified:10 Nov 2021 16:33

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