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Real-time detection of an airborne microorganism using inertial impaction and mini-fluorescent microscopy

Kang, Joon Sang and Lee, Kang Soo and Kim, Sang Soo and Bae, Gwi-Nam and Jung, Jae Hee (2013) Real-time detection of an airborne microorganism using inertial impaction and mini-fluorescent microscopy. Lab on a Chip, 14 (1). pp. 244-251. ISSN 1473-0197.

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To achieve successful real-time detection of airborne pathogenic microorganisms, the problem must be considered in terms of their physical size and biological characteristics. We developed an airborne microorganism detection chip to realize the detection of microorganisms, ensuring compactness, sensitivity, cost-efficiency, and portability, using three key components: an inertial impaction system, a cartridge-type impaction plate, and a mini-fluorescent microscope. The inertial impaction system was used to separate microorganisms in terms of their aerodynamic particle size, and was fabricated with three impaction stages. Numerical analysis was performed to design the system; the calculated cutoff diameter at each impaction stage was 2.02 (first stage), 0.88 (second stage), and 0.54 μm (third stage). The measured cutoff diameters were 2.24, 0.91, and 0.49 μm, respectively. A cartridge-type impaction plate was used, composed of molded polydimethylsiloxane (PDMS) and an actual impaction region made of a SYBR green I dye-stained agar plate. A mini-fluorescent microscope was used to distinguish microbes from non-biological particles. Images of the microorganisms deposited at the impaction zone were obtained via mini-fluorescent microscopy, and fluorescent intensities of the images were calculated using in-house image-processing software. The results showed that the developed system successfully identified aerosolized biological particles from non-biological particles in real time.

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Additional Information:© 2014 The Royal Society of Chemistry. Received 7th July 2013, Accepted 30th September 2013. First published online 02 Oct 2013. This research was supported by the Converging Research Center Program funded by the Ministry of Education, Science, and Technology (2012K001370) and was partially supported by the KIST Institutional Program (2E23972).
Funding AgencyGrant Number
Converging Research Center ProgramUNSPECIFIED
Ministry of Education, Science, and Technology (Korea)2012K001370
KIST Institutional Program2E23972
Issue or Number:1
Record Number:CaltechAUTHORS:20140109-092026076
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:43287
Deposited By: Tony Diaz
Deposited On:15 Jan 2014 16:16
Last Modified:14 Dec 2019 00:17

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