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Comprehensive proteomic profiling of outer membrane vesicles from Campylobacter jejuni

Jang, Kyoung-Soon and Sweredoski, Michael J. and Graham, Robert L. J. and Hess, Sonja and Clemons, William M., Jr. (2014) Comprehensive proteomic profiling of outer membrane vesicles from Campylobacter jejuni. Journal of Proteomics, 98 . pp. 90-98. ISSN 1874-3919. PMCID PMC4534003. http://resolver.caltech.edu/CaltechAUTHORS:20140110-082848696

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Abstract

Gram-negative bacteria constitutively release outer membrane vesicles (OMVs) during cell growth that play significant roles in bacterial survival, virulence and pathogenesis. In this study, comprehensive proteomic analysis of OMVs from a human gastrointestinal pathogen Campylobacter jejuni NCTC11168 was performed using high-resolution mass spectrometry. The OMVs of C. jejuni NCTC11168 were isolated from culture supernatants then characterized using electron microscopy and dynamic light scattering revealing spherical OMVs of an average diameter of 50 nm. We then identified 134 vesicular proteins using high-resolution LTQ-Orbitrap mass spectrometry. Subsequent functional analysis of the genes revealed the relationships of the vesicular proteins. Furthermore, known N-glycoproteins were identified from the list of the vesicular proteome, implying the potential role of the OMVs as a delivery means for biologically relevant bacterial glycoproteins. These results enabled us to elucidate the overall proteome profile of pathogenic bacterium C. jejuni and to speculate on the function of OMVs in bacterial infections and communication.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.jprot.2013.12.014DOIArticle
http://www.sciencedirect.com/science/article/pii/S1874391913006490PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4534003/PubMed CentralArticle
ORCID:
AuthorORCID
Sweredoski, Michael J.0000-0003-0878-3831
Hess, Sonja0000-0002-5904-9816
Clemons, William M., Jr.0000-0002-0021-889X
Additional Information:© 2013 Elsevier B.V. Received 12 April 2013; Accepted 17 December 2013; Available online 29 December 2013. We thank Sarkis Mazmanian for providing support for bacterial culture growth, Alasdair McDowall in the Cryo-electron Microscopy Facility at the Broad Center for the help with electron microscopy, and Axel Müller for optimizing the bacterial growth conditions (all at Caltech). We also thank V. Somalinga and A. Müller for their critical reading of our paper. This work was supported by a Searle Scholar Fellowship and a Burroughs-Wellcome Fund Career Award to WMC. The PEL is supported by the Gordon and Betty Moore Foundation through Grant 442 GBMF775 and the Beckman Institute.
Funders:
Funding AgencyGrant Number
Searle Scholars ProgramUNSPECIFIED
Burroughs-Wellcome FundUNSPECIFIED
Gordon and Betty Moore FoundationGBMF775
Caltech Beckman InstituteUNSPECIFIED
Subject Keywords:C. jejuni NCTC11168; Functional annotation; Outer membrane vesicle; Signal peptide; Virulence factor
PubMed Central ID:PMC4534003
Record Number:CaltechAUTHORS:20140110-082848696
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20140110-082848696
Official Citation:Kyoung-Soon Jang, Michael J. Sweredoski, Robert L.J. Graham, Sonja Hess, William M. Clemons Jr., Comprehensive proteomic profiling of outer membrane vesicles from Campylobacter jejuni, Journal of Proteomics, Volume 98, 26 February 2014, Pages 90-98, ISSN 1874-3919, http://dx.doi.org/10.1016/j.jprot.2013.12.014. (http://www.sciencedirect.com/science/article/pii/S1874391913006490)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:43302
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:15 Jan 2014 00:12
Last Modified:20 Jul 2017 22:15

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