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Application of Nanoparticle Antioxidants to Enable Hyperstable Chloroplasts for Solar Energy Harvesting

Boghossian, Ardernis A. and Sen, Fatih and Gibbons, Brenna M. and Sen, Selda and Faltermeier, Sean M. and Giraldo, Juan Pablo and Zhang, Cathy T. and Zhang, Jingqing and Heller, Daniel A. and Strano, Michael S. (2013) Application of Nanoparticle Antioxidants to Enable Hyperstable Chloroplasts for Solar Energy Harvesting. Advanced Energy Materials, 3 (7). pp. 881-893. ISSN 1614-6832. doi:10.1002/aenm.201201014.

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The chloroplast contains densely stacked arrays of light-harvesting proteins that harness solar energy with theoretical maximum glucose conversion efficiencies approaching 12%. Few studies have explored isolated chloroplasts as a renewable, abundant, and low cost source for solar energy harvesting. One impediment is that photoactive proteins within the chloroplast become photodamaged due to reactive oxygen species (ROS) generation. In vivo, chloroplasts reduce photodegradation by applying a self-repair cycle that dynamically replaces photodamaged components; outside the cell, ROS-induced photodegradation contributes to limited chloroplast stability. The incorporation of chloroplasts into synthetic, light-harvesting devices will require regenerative ROS scavenging mechanisms to prolong photoactivity. Herein, we study ROS generation within isolated chloroplasts extracted from Spinacia oleracea directly interfaced with nanoparticle antioxidants, including dextran-wrapped nanoceria (dNC) previously demonstrated as a potent ROS scavenger. We quantitatively examine the effect of dNC, along with cerium ions, fullerenol, and DNA-wrapped single-walled carbon nanotubes (SWCNTs), on the ROS generation of isolated chloroplasts using the oxidative dyes, 2’,7’- dichlorodihydrofluorescein diacetate (H_2DCF-DA) and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide sodium salt (XTT). Electrochemical measurements confirm that chloroplasts processed from free solution can generate power under illumination. We find dNC to be the most effective of these agents for decreasing oxidizing species and superoxide concentrations whilst preserving chloroplast photoactivity at concentrations below 5 μM, offering a promising mechanism for maintaining regenerative chloroplast photoactivity for light-harvesting applications.

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Additional Information:© 2013 Wiley-VCH Verlag GmbH & Co. Received: December 3, 2012. Article first published online: 15 Mar 2013. This work was financially supported by a grant from the U.S. Department of Energy (grant no. ER46488). A.A.B. is grateful for support from the National Defense Science and Engineering Graduate (NDSEG) Fellowship. F.S and S.S. thank TUBITAK for the 2211 and 2214-Research fellowship program and the METU-DPT-OYP program. S.M.F. is grateful for support from the National Science Foundation (NSF) Graduate Fellowship. This material is based upon work supported by the National Science Foundation Postdoctoral Research Fellowship in Biology (J.P.G.) under Grant No. 1103600.
Funding AgencyGrant Number
Department of Energy (DOE)ER46488
National Defense Science and Engineering Graduate (NDSEG) FellowshipUNSPECIFIED
NSF Graduate Research FellowshipUNSPECIFIED
NSF Postdoctoral FellowshipDBI-1103600
Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK)UNSPECIFIED
Subject Keywords: dextran-wrapped nanoceria; cerium oxide (CeO2) nanoparticle; single-walled carbon nanotube (SWCNT); fullerenol (polyhydroxylated fullerene; buckeyball; C60); 2′,7′-dichlorodihydrofluorescein diacetate (H2DCF-DA); 2, 3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide sodium salt (XTT); dichloroindophenol (DCPIP); reactive oxygen species (ROS); regeneration; scavenging; chloroplast
Issue or Number:7
Record Number:CaltechAUTHORS:20140113-101809542
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Official Citation:Boghossian, A. A., Sen, F., Gibbons, B. M., Sen, S., Faltermeier, S. M., Giraldo, J. P., Zhang, C. T., Zhang, J., Heller, D. A. and Strano, M. S. (2013), Application of Nanoparticle Antioxidants to Enable Hyperstable Chloroplasts for Solar Energy Harvesting. Adv. Energy Mater., 3: 881–893. doi: 10.1002/aenm.201201014
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:43339
Deposited By: Ruth Sustaita
Deposited On:13 Jan 2014 18:45
Last Modified:10 Nov 2021 16:36

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