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Genome of the human hookworm Necator americanus

Tang, Yat T. and Gao, Xin and Rosa, Bruce A. and Sternberg, Paul W. (2014) Genome of the human hookworm Necator americanus. Nature Genetics, 46 (3). pp. 261-269. ISSN 1061-4036. PMCID PMC3950333. http://resolver.caltech.edu/CaltechAUTHORS:20140127-101549521

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Abstract

The hookworm Necator americanus is the predominant soil-transmitted human parasite. Adult worms feed on blood in the small intestine, causing iron-deficiency anemia, malnutrition, growth and development stunting in children, and severe morbidity and mortality during pregnancy in women. We report sequencing and assembly of the N. americanus genome (244 Mb, 1 19,151 1 genes). Characterization of this first hookworm genome sequence identified genes orchestrating the hookworm’s invasion of the human host, genes involved in blood feeding and development, and genes encoding proteins that represent new potential drug targets against hookworms. N. americanus has undergone a considerable and unique expansion of immunomodulator proteins, some of which we highlight as potential treatments against inflammatory diseases. We also used a protein microarray to demonstrate a postgenomic application of the hookworm genome sequence. This genome provides an invaluable resource to boost ongoing efforts toward fundamental and applied postgenomic research, including the development of new methods to control hookworm and human immunological diseases.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/ng.2875DOIArticle
http://www.nature.com/ng/journal/v46/n3/full/ng.2875.htmlPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3950333/PubMed CentralArticle
http://rdcu.be/cmP9PublisherFree ReadCube access
ORCID:
AuthorORCID
Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 2013 Nature Publishing Group, a division of Macmillan Publishers Limited. This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/. Received 10 June 2013; accepted 18 December 2013; published online 19 January 2014. We thank the faculty and staff of the Genome Institute at Washington University and the Protein Microarray Laboratory at the University of California–Irvine (U54AI065359) who contributed to this study. The genome sequencing and annotation work was funded by US National Institutes of Health (NIH)–National Human Genome Research Institute grant U54HG003079 to R.K.W. Comparative genome analysis was funded by grants NIH–National Institute of Allergy and Infectious Diseases AI081803 and NIH–National Institute of General Medical Sciences GM097435 to M.M. Funds from the Australian Research Council and Australia’s National Health and Medical Research Council to R.B.G. are gratefully acknowledged. P.W.S. is an investigator with the Howard Hughes Medical Institute. We thank the faculty and staff of The Genome Institute at Washington University who contributed to this study. These authors contributed equally to this work: Yat T Tang, Xin Gao & Bruce A Rosa Author Contributions: Y.T.T., X.G. and B.A.R. contributed equally to this work. M.M., R.B.G., P.W.S., R.K.W. and S.R. conceived and planned the project. M.M. led the project, analysis and manuscript preparation. B.Z., P.J.H., J.M.H., P.L.F., J.B. and E.M.R. provided material. K.H.-P., X.Z., V.B.-P., P.M., W.C.W., J. Martin and S.A. produced sequence data and constructed, annotated and submitted the assembly. M.M., Y.T.T., X.G., B.A.R., R.T., Q.W., S.A., J. Martin, E.H., A.L., S.T.G., P.L.F., J. Mulvenna, J.S. and A.D. performed genome-based comparative studies, differential transcription, host-parasite interaction analysis, and proteomics and protein-array analysis. M.M., R.B.G., A.L. and J.M.H. drafted, edited and wrote the manuscript. The authors declare no competing financial interests.
Funders:
Funding AgencyGrant Number
NIHU54HG003079
NIHAI081803
NIHGM097435
Australian Research CouncilUNSPECIFIED
National Health and Medical Research Council (NHMRC)UNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
PubMed Central ID:PMC3950333
Record Number:CaltechAUTHORS:20140127-101549521
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20140127-101549521
Official Citation:Genome of the human hookworm Necator americanus Yat T Tang et al. Nature Genetics 46, 261–269 (2014) doi:10.1038/ng.2875
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:43523
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:05 Feb 2014 20:43
Last Modified:08 Sep 2016 16:33

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