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Biosynthesis of the actinomycin chromophore: incorporation of 3-hydroxy-4-methylanthranilic acid into actinomycins by streptomyces antibioticus

Golub, Ellis E. and Ward, Marth A. and Nishimura, Jonathan S. (1969) Biosynthesis of the actinomycin chromophore: incorporation of 3-hydroxy-4-methylanthranilic acid into actinomycins by streptomyces antibioticus. Journal of Bacteriology, 100 (2). pp. 977-984. ISSN 0021-9193. PMCID PMC250184. https://resolver.caltech.edu/CaltechAUTHORS:GOLbact69

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Abstract

Actinomycin synthesis by washed mycelia of Streptomyces antibioticus has been conducted in the presence of 3-hydroxy-4-methylanthranilate-(carboxyl-14C). Incorporation of this compound into actinomycins has been observed, which constitutes further evidence that 3-hydroxy-4-methylanthranilate is an intermediate in actinomycin biosynthesis. The position of the incorporated label has been determined to be within the actinomycin chromophore, and the label appears to be equally distributed between both halves of the chromophore. Incidental to these findings was the observation that the 14C-labeled actinomycins were subject to rapid reabsorption by the organism with actinomycin V taken up preferentially to actinomycin IV.


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Additional Information:© 1969 American Society for Microbiology. Received for publication 12 August 1969. The authors wish to thank Steven H. Weinograd for skillful technical assistance. This investigation was supported by Public Health Service grant AI-06366 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, grant GB8083 from the National Science Foundation, and the Tufts University Cancer Committee.
Funders:
Funding AgencyGrant Number
NIHAI-06366
NIHGB8083
Tufts UniversityUNSPECIFIED
Issue or Number:2
PubMed Central ID:PMC250184
Record Number:CaltechAUTHORS:GOLbact69
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:GOLbact69
Official Citation:Biosynthesis of the Actinomycin Chromophore: Incorporation of 3-Hydroxy-4-Methylanthranilic Acid into Actinomycins by Streptomyces antibioticus Ellis E. Golub, Martha A. Ward, Jonathan S. Nishimura Journal of Bacteriology Nov 1969, 100 (2) 977-984
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4372
Collection:CaltechAUTHORS
Deposited By: Lindsay Cleary
Deposited On:18 Aug 2006
Last Modified:02 Oct 2019 23:12

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