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Analysis of the transcriptome of adult Dictyocaulus filaria and comparison with Dictyocaulus viviparus, with a focus on molecules involved in host–parasite interactions

Mangiola, Stefano and Young, Neil D. and Sternberg, Paul W. and Strube, Christina and Korhonen, Pasi K. and Mitreva, Makedonka and Scheerlinck, Jean-Pierre and Hofmann, Andreas and Jex, Aaron R. and Gasser, Robin B. (2014) Analysis of the transcriptome of adult Dictyocaulus filaria and comparison with Dictyocaulus viviparus, with a focus on molecules involved in host–parasite interactions. International Journal for Parasitology, 44 (3-4). pp. 251-261. ISSN 0020-7519. PMCID PMC4040346. doi:10.1016/j.ijpara.2013.12.003.

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Parasitic nematodes cause diseases of major economic importance in animals. Key representatives are species of Dictyocaulus (=lungworms), which cause bronchitis (=dictyocaulosis, commonly known as “husk”) and have a major adverse impact on the health of livestock. In spite of their economic importance, very little is known about the immunomolecular biology of these parasites. Here, we conducted a comprehensive investigation of the adult transcriptome of Dictyocaulus filaria of small ruminants and compared it with that of Dictyocaulus viviparus of bovids. We then identified a subset of highly transcribed molecules inferred to be linked to host–parasite interactions, including cathepsin B peptidases, fatty-acid and/or retinol-binding proteins, β-galactoside-binding galectins, secreted protein 6 precursors, macrophage migration inhibitory factors, glutathione peroxidases, a transthyretin-like protein and a type 2-like cystatin. We then studied homologues of D. filaria type 2-like cystatin encoded in D. viviparus and 24 other nematodes representing seven distinct taxonomic orders, with a particular focus on their proposed role in immunomodulation and/or metabolism. Taken together, the present study provides new insights into nematode–host interactions. The findings lay the foundation for future experimental studies and could have implications for designing new interventions against lungworms and other parasitic nematodes. The future characterisation of the genomes of Dictyocaulus spp. should underpin these endeavours.

Item Type:Article
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Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 2014 Australian Society for Parasitology Inc. Published by Elsevier Ltd. Received 1 October 2013; Received in revised form 11 December 2013; Accepted 18 December 2013; Available online 31 January 2014. This project was funded by the Australian Research Council (ARC) and Australian National Health & Medical Research Council (NHMRC). This project was also supported by a Victorian Life Sciences Computation Initiative (VLSCI) grant number VR0007 on its Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government, Australia. Other support from the Alexander von Humboldt Foundation, Germany, and the Melbourne Water Corporation, Australia, is gratefully acknowledged (R.B.G), as is funding from and the Howard Hughes Medical Institute (HHMI), USA and National Institutes of Health (NIH), USA (P.W.S.). M.M. also received funds from NIH. N.D.Y is an NHMRC Early Career Research (ECR) Fellow. We also acknowledge the continued contributions of staff at WormBase (
Funding AgencyGrant Number
Australian Research CouncilUNSPECIFIED
Australian National Health and Medical Research Council (NHMRC)UNSPECIFIED
Victorian Life Sciences Computation Initiative (VLSCI)VR0007
Alexander von Humboldt FoundationUNSPECIFIED
Melbourne Water CorporationUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Subject Keywords: Lungworms; Dictyocaulus spp.; Transcriptome; Host–parasite interactions
Issue or Number:3-4
PubMed Central ID:PMC4040346
Record Number:CaltechAUTHORS:20140210-115459871
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Official Citation:Stefano Mangiola, Neil D. Young, Paul W. Sternberg, Christina Strube, Pasi K. Korhonen, Makedonka Mitreva, Jean-Pierre Scheerlinck, Andreas Hofmann, Aaron R. Jex, Robin B. Gasser, Analysis of the transcriptome of adult Dictyocaulus filaria and comparison with Dictyocaulus viviparus, with a focus on molecules involved in host–parasite interactions, International Journal for Parasitology, Available online 31 January 2014, ISSN 0020-7519, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:43743
Deposited By: Ruth Sustaita
Deposited On:10 Feb 2014 21:04
Last Modified:10 Nov 2021 16:42

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