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A Reporter Assay in Lamprey Embryos Reveals Both Functional Conservation and Elaboration of Vertebrate Enhancers

Parker, Hugo J. and Sauka-Spengler, Tatjana and Bronner, Marianne and Elgar, Greg (2014) A Reporter Assay in Lamprey Embryos Reveals Both Functional Conservation and Elaboration of Vertebrate Enhancers. PLoS ONE, 9 (1). Art. No. e85492. ISSN 1932-6203. PMCID PMC3887057. https://resolver.caltech.edu/CaltechAUTHORS:20140214-093316457

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Abstract

The sea lamprey is an important model organism for investigating the evolutionary origins of vertebrates. As more vertebrate genome sequences are obtained, evolutionary developmental biologists are becoming increasingly able to identify putative gene regulatory elements across the breadth of the vertebrate taxa. The identification of these regions makes it possible to address how changes at the genomic level have led to changes in developmental gene regulatory networks and ultimately to the evolution of morphological diversity. Comparative genomics approaches using sea lamprey have already predicted a number of such regulatory elements in the lamprey genome. Functional characterisation of these sequences and other similar elements requires efficient reporter assays in lamprey. In this report, we describe the development of a transient transgenesis method for lamprey embryos. Focusing on conserved non-coding elements (CNEs), we use this method to investigate their functional conservation across the vertebrate subphylum. We find instances of both functional conservation and lineage-specific functional evolution of CNEs across vertebrates, emphasising the utility of functionally testing homologous CNEs in their host species.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1371/journal.pone.0085492DOIArticle
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0085492PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3887057/PubMed CentralArticle
ORCID:
AuthorORCID
Parker, Hugo J.0000-0001-7646-2007
Sauka-Spengler, Tatjana0000-0001-9289-0263
Bronner, Marianne0000-0003-4274-1862
Additional Information:© 2014 Parker et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received: August 19, 2013; Accepted: December 5, 2013; Published: January 9, 2014. This work was supported by MRC core funding (U117597141) to GE and grants GM090049 and DE017911 to MEB. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank Natalya Nikitina, Benjamin Uy, Melinda Modrell and Marcos Simoes-Costa for advice and assistance on lamprey husbandry. We thank Heather Callaway for zebrafish maintenance. Author Contributions: Conceived and designed the experiments: HP GE TSS MB. Performed the experiments: HP TSS. Analyzed the data: HP. Contributed reagents/ materials/analysis tools: GE MB. Wrote the paper: HP GE.
Funders:
Funding AgencyGrant Number
Medical Research Council (MRC)U117597141
NIHGM090049
NIHDE017911
Issue or Number:1
PubMed Central ID:PMC3887057
Record Number:CaltechAUTHORS:20140214-093316457
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20140214-093316457
Official Citation:Parker HJ, Sauka-Spengler T, Bronner M, Elgar G (2014) A Reporter Assay in Lamprey Embryos Reveals Both Functional Conservation and Elaboration of Vertebrate Enhancers. PLoS ONE 9(1): e85492. doi:10.1371/journal.pone.0085492
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:43832
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:18 Feb 2014 15:54
Last Modified:09 Mar 2020 13:19

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