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Encoding regulatory state boundaries in the pregastrular oral ectoderm of the sea urchin embryo

Li, Enhu and Cui, Miao and Peter, Isabelle S. and Davidson, Eric H. (2014) Encoding regulatory state boundaries in the pregastrular oral ectoderm of the sea urchin embryo. Proceedings of the National Academy of Sciences of the United States of America, 111 (10). E906-E913. ISSN 0027-8424. PMCID PMC3956148. https://resolver.caltech.edu/CaltechAUTHORS:20140225-091728610

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Abstract

By gastrulation the ectodermal territories of the sea urchin embryo have developed an unexpectedly complex spatial pattern of sharply bounded regulatory states, organized orthogonally with respect to the animal/vegetal and oral/aboral axes of the embryo. Although much is known of the gene regulatory network (GRN) linkages that generate these regulatory states, the principles by which the boundaries between them are positioned and maintained have remained undiscovered. Here we determine the encoded genomic logic responsible for the boundaries of the oral aspect of the embryo that separate endoderm from ectoderm and ectoderm from neurogenic apical plate and that delineate the several further subdivisions into which the oral ectoderm per se is partitioned. Comprehensive regulatory state maps, including all spatially expressed oral ectoderm regulatory genes, were established. The circuitry at each boundary deploys specific repressors of regulatory states across the boundary, identified in this work, plus activation by broadly expressed positive regulators. These network linkages are integrated with previously established interactions on the oral/aboral axis to generate a GRN model encompassing the 2D organization of the regulatory state pattern in the pregastrular oral ectoderm of the embryo.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.1323105111DOIArticle
http://www.pnas.org/content/111/10/E906.longPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3956148/PubMed CentralArticle
ORCID:
AuthorORCID
Peter, Isabelle S.0000-0003-3685-3147
Additional Information:© 2014 National Academy of Sciences. Contributed by Eric H. Davidson, January 27, 2014 (sent for review January 1, 2014); Published ahead of print February 20, 2014. We thank Prof. Robert Burke (University of Victoria) and Prof. Richard Harland (University of California, Berkeley) for their very helpful critical reviews of this paper. Research was supported by National Institutes of Health Grant HD-037105 and by the Lucille P. Markey Charitable Trust. Author contributions: E.L., M.C., I.S.P., and E.H.D. designed research; E.L. and M.C. performed research; E.L., M.C., I.S.P., and E.H.D. analyzed data; and E.L., M.C., I.S.P., and E.H.D. wrote the paper. The authors declare no conflict of interest.
Funders:
Funding AgencyGrant Number
NIHHD-37105
Lucille P. Markey Charitable TrustUNSPECIFIED
Subject Keywords:regulatory state boundaries; pattern formation; repression circuitry
Issue or Number:10
PubMed Central ID:PMC3956148
Record Number:CaltechAUTHORS:20140225-091728610
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20140225-091728610
Official Citation:Li, E., Cui, M., Peter, I. S., & Davidson, E. H. (2014). Encoding regulatory state boundaries in the pregastrular oral ectoderm of the sea urchin embryo. Proceedings of the National Academy of Sciences, 111(10), E906-E913. doi: 10.1073/pnas.1323105111
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:43972
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:28 Feb 2014 16:14
Last Modified:03 Oct 2019 06:13

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