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Reactivation of ethanol-calcium-isolated cilia from Tetrahymena pyriformis

Winicur, Sandra (1967) Reactivation of ethanol-calcium-isolated cilia from Tetrahymena pyriformis. Journal of Cell Biology, 35 (1). C7-C9. ISSN 0021-9525. PMCID PMC2107110. doi:10.1083/jcb.35.1.c7.

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Cilia isolated from Tetrahymena after ciliary shedding is induced by the addition of CaCl2 or other salts to cells suspended in an ethanol solution (4, 6, 11) have been studied chemically and enzymatically (4, 6, 11, 12, 13), but until now have never been shown to be capable of motility in the presence of ATP. In contrast to this, Gibbons (7) has demonstrated the reactivation of glycerol-isolated cilia by techniques similar to those developed for grasshopper sperm tails (8) and Polytoma flagella (2). However, the glycerol technique is much less satisfactory than the ethanol-calcium method for isolating pure cilia. This paper describes the reactivation, which can be obtained with ATP or ADP, of cilia isolated by an ethanol-calcium method (6), followed by glycerination or other treatments.

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Additional Information:© 1967 by Rockefeller University Press Received for publication 3 July 1967. This work was supported by United States Public Health Service Training Grant 5 T1 GM-86. I would like to thank Dr. Charles J. Brokaw for his continued help and advice, Mr. Peter N. Cross for his valuable assistance in some of the experiments, and Dr. Michael Holwill for the use of his temperature-regulated microscope apparatus.
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NIH Predoctoral Fellowship5 T1 GM-86
Issue or Number:1
PubMed Central ID:PMC2107110
Record Number:CaltechAUTHORS:WINjcb67
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4408
Deposited By: Archive Administrator
Deposited On:22 Aug 2006
Last Modified:08 Nov 2021 20:17

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