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Studies on the denaturation of antibody: I. The action of urea on diphtheria antitoxin

Wright, George G. (1944) Studies on the denaturation of antibody: I. The action of urea on diphtheria antitoxin. Journal of Experimental Medicine, 79 (4). pp. 455-461. ISSN 0022-1007. PMCID PMC2135386. doi:10.1084/jem.79.4.455.

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Although a great deal of information has been accumulated regarding the stability of antibody under various conditions and treatments (1), the action of urea and similar substamces seems to have been largely neglected. Since the action of urea in denaturing proteins involves weakening the secondary bonds about the peptide linkages (2, 3) it is of interest to know the effect of urea on antibody. Pappenheimer, Lundgren, and Williams (4) report that diphtheria antitoxin is unaffected by "strong" urea solutions. Very recently Erickson and Neurath (5) have reported some experiments on the action of guanidine hydrochloride on horse antipneumococcus antibody. The present investigation of the stability of antibody in urea was undertaken because of the presumable importance of the results to the theory of antibody structure. Diphtheria antitoxin has been chosen as the antibody for study because the accurate neutralization methods available for its determination permit direct measurement of the fundamental property of antibody, its ability to combine with antigen.

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Additional Information:© 1944, by The Rockefeller Institute for Medical Research New York. (Received for publication, December 18, 1943) This investigation was carried out with the aid of a grant from The Rockefeller Foundation. The author is indebted to Professor Linus Pauling, Dr. Henry B. Bull, Dr. Dan H. Campbell, and other associates in these Laboratories for valuable suggestions and discussion. [G.G.W. was a] Fellow of the Medical Fellowship Board of the National Research Council. Contribution No. 952 from the Gates and Crellin Laboratories of Chemistry, California Institute of Technology, Pasadena
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National Research CouncilUNSPECIFIED
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Gates and Crellin Laboratories of Chemistry952
Issue or Number:4
PubMed Central ID:PMC2135386
Record Number:CaltechAUTHORS:WRIjem44
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4416
Deposited By: Archive Administrator
Deposited On:22 Aug 2006
Last Modified:08 Nov 2021 20:17

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