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Global molecular analyses of methane metabolism in methanotrophic Alphaproteobacterium, Methylosinus trichosporium OB3b. Part II. metabolomics and 13C-labeling study

Yang, Song and Matsen, Janet B. and Kunopka, Michael and Green-Saxena, Abigail and Clubb, Justin and Sadilek, Martin and Orphan, Victoria J. and Beck, David and Kalyuzhnaya, Marina G. (2013) Global molecular analyses of methane metabolism in methanotrophic Alphaproteobacterium, Methylosinus trichosporium OB3b. Part II. metabolomics and 13C-labeling study. Frontiers in Microbiology, 4 . Art. No. 70. ISSN 1664-302X. PMCID PMC3615224. https://resolver.caltech.edu/CaltechAUTHORS:20140310-084832957

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Abstract

In this work we use metabolomics and ^(13)C-labeling data to refine central metabolic pathways for methane utilization in Methylosinus trichosporium OB3b, a model alphaproteobacterial methanotrophic bacterium. We demonstrate here that similar to non-methane utilizing methylotrophic alphaproteobacteria the core metabolism of the microbe is represented by several tightly connected metabolic cycles, such as the serine pathway, the ethylmalonyl-CoA (EMC) pathway, and the citric acid (TCA) cycle. Both in silico estimations and stable isotope labeling experiments combined with single cell (NanoSIMS) and bulk biomass analyses indicate that a significantly larger portion of the cell carbon (over 60%) is derived from CO_2 in this methanotroph. Our ^(13) C-labeling studies revealed an unusual topology of the assimilatory network in which phosph(enol) pyruvate/pyruvate interconversions are key metabolic switches. A set of additional pathways for carbon fixation are identified and discussed.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.3389/fmicb.2013.00070DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3615224/PubMed CentralArticle
http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00070/fullPublisherArticle
ORCID:
AuthorORCID
Green-Saxena, Abigail0000-0002-8502-6589
Orphan, Victoria J.0000-0002-5374-6178
Kalyuzhnaya, Marina G.0000-0002-9058-7794
Additional Information:© 2013 Yang, Matsen, Konopka, Green-Saxena, Clubb, Sadilek, Orphan, Beck and Kalyuzhnaya. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc. Received: 01 January 2013; paper pending published: 28 January 2013; accepted: 12 March 2013; published online: 03 April 2013. We are very grateful to Dr. Mary E. Lidstrom, Dr. Ivan Bergand Dr. Ludmila Chistoserdova for insightful suggestions on the manuscript. This work was supported by the DOE (DE-SC0005154).
Funders:
Funding AgencyGrant Number
Department of Energy (DOE)DE-SC0005154
Subject Keywords:methanotrophic proteobacteria, serine cycle, ethylmalonyl-CoA pathway in methanotrophs, metabolic fluxes labeling
PubMed Central ID:PMC3615224
Record Number:CaltechAUTHORS:20140310-084832957
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20140310-084832957
Official Citation:Global Molecular Analyses of Methane Metabolism in Methanotrophic Alphaproteobacterium, Methylosinus trichosporium OB3b. Part II. Metabolomics and 13C-Labeling Study Song Yang, Janet B. Matsen, Michael Konopka, Abigail Green-Saxena, Justin Clubb, Martin Sadilek, Victoria J. Orphan, David Beck, Marina G. Kalyuzhnaya Front Microbiol. 2013; 4: 70. Prepublished online Jan 28, 2013. Published online Apr 3, 2013. doi: 10.3389/fmicb.2013.00070 PMCID: PMC3615224
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:44207
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:10 Mar 2014 16:18
Last Modified:03 Oct 2019 19:47

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