CaltechAUTHORS
  A Caltech Library Service

Computer Simulation of Flagellar Movement X. Doublet pair splitting and bend propagation modeled using stochastic dynein kinetics

Brokaw, Charles J. (2014) Computer Simulation of Flagellar Movement X. Doublet pair splitting and bend propagation modeled using stochastic dynein kinetics. Cytoskeleton . ISSN 1949-3584. https://resolver.caltech.edu/CaltechAUTHORS:20140310-091040155

[img]
Preview
PDF - Accepted Version
See Usage Policy.

577Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20140310-091040155

Abstract

Experimental observations on cyclic splitting and bending by a flagellar doublet pair are modeled using forces obtained from a model for dynein mechanochemistry, based on ideas introduced by Andrew Huxley and Terrill Hill and extended previously for modeling flagellar movements. The new feature is elastic attachment of dynein to the A doublet, which allows movement perpendicular to the A doublet and provides adhesive force that can strain attached dyneins. This additional strain influences the kinetics of dynein attachment and detachment. Computations using this dynein model demonstrate that very simple and realistic ideas about dynein mechanochemistry are sufficient for explaining the separation and reattachment seen experimentally with flagellar doublet pairs. Additional simulations were performed after adding a “super-adhesion” elasticity. This elastic component is intended to mimic interdoublet connections, normally present in an intact axoneme, that would prevent visible splitting but allow sufficient separation to cause dynein detachment and cessation of shear force generation. This is the situation envisioned by Lindemann's “geometric clutch” hypothesis for control of dynein function in flagella and cilia. The simulations show abrupt disengagement of the “clutch” at one end of a bend, and abrupt reengagement of the “clutch” at the other end of a bend, ensuring that active sliding is only operating where it will cause bend propagation from base to tip.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://onlinelibrary.wiley.com/doi/10.1002/cm.21168/abstractPublisherArticle
http://dx.doi.org/10.1002/cm.21168DOIArticle
Additional Information:© 2014 Wiley-Blackwell, Inc. This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process which may lead to differences between this version and the Version of Record.
Subject Keywords: axoneme; buckling; cilia; motility; oscillation
Record Number:CaltechAUTHORS:20140310-091040155
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20140310-091040155
Official Citation:Computer simulation of flagellar movement X. doublet pair splitting and bend propagation modeled using stochastic dynein kinetics Charles J. Brokaw Accepted manuscript online: 25 FEB 2014 03:43AM EST | DOI: 10.1002/cm.21168
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:44208
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:10 Mar 2014 17:48
Last Modified:03 Oct 2019 06:15

Repository Staff Only: item control page