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Identification of germline transcriptional regulatory elements in Aedes aegypti

Akbari, Omar S. and Papathanos, Philippos A. and Sandler, Jeremy E. and Kennedy, Katie and Hay, Bruce A. (2014) Identification of germline transcriptional regulatory elements in Aedes aegypti. Scientific Reports, 4 . Art. No. 3954. ISSN 2045-2322. PMCID PMC3912481. http://resolver.caltech.edu/CaltechAUTHORS:20140317-110101980

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Abstract

The mosquito Aedes aegypti is the principal vector for the yellow fever and dengue viruses, and is also responsible for recent outbreaks of the alphavirus chikungunya. Vector control strategies utilizing engineered gene drive systems are being developed as a means of replacing wild, pathogen transmitting mosquitoes with individuals refractory to disease transmission, or bringing about population suppression. Several of these systems, including Medea, UD^MEL, and site-specific nucleases, which can be used to drive genes into populations or bring about population suppression, utilize transcriptional regulatory elements that drive germline-specific expression. Here we report the identification of multiple regulatory elements able to drive gene expression specifically in the female germline, or in the male and female germline, in the mosquito Aedes aegypti. These elements can also be used as tools with which to probe the roles of specific genes in germline function and in the early embryo, through overexpression or RNA interference.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/srep03954DOIArticle
http://www.nature.com/srep/2014/140204/srep03954/full/srep03954.htmlPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3912481/PubMed CentralArticle
Additional Information:© 2014 Macmillan Publishers Limited.. This work is licensed under a Creative Commons Attribution- NonCommercial-NoDerivs 3.0 Unported license. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0. Received: 20 September 2013; Accepted: 16 January 2014; Published: 04 February 2014. This work was supported by grants awarded to B.A.H. by NIH (DPI OD003878). This work was supported by the U. S. Army Research Laboratory and the U. S. Army Research Office under contract W911NF-11-2-0055 to the California Institute of Technology, and by a grant to BAH from the NIH (DP1 OD003878). Author contributions: O.S.A., P.P. and B.A.H. designed research; O.S.A., P.P., K.K. and J.S. performed experiments; O.S.A., P.P. and J.S. analyzed data, O.S.A., P.P. and B.A.H. discussed results; O.S.A., P.P. and B.A.H. wrote the manuscript with help from all authors.
Funders:
Funding AgencyGrant Number
NIHDPI OD003878
Army Research LaboratoryUNSPECIFIED
Army Research Office (ARO)W911NF-11-2-0055
Subject Keywords:Genomic engineering; Genetic engineering
PubMed Central ID:PMC3912481
Record Number:CaltechAUTHORS:20140317-110101980
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20140317-110101980
Official Citation:Akbari, O.S., Papathanos, P.A., Sandler, J., Kennedy, K. &Hay, B.A. Identification of germline transcriptional regulatory elements in Aedes aegypti. Sci. Rep. 4, 3954; DOI:10.1038/srep03954 (2014).
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:44349
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:19 Mar 2014 23:13
Last Modified:25 Jul 2017 19:35

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