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Architecture and host interface of environmental chlamydiae revealed by electron cryotomography

Pilhofer, Martin and Aistleitner, Karin and Ladinsky, Mark S. and König, Lena and Horn, Matthias and Jensen, Grant J. (2014) Architecture and host interface of environmental chlamydiae revealed by electron cryotomography. Environmental Microbiology, 16 (2). pp. 417-429. ISSN 1462-2912. PMCID PMC4949044. http://resolver.caltech.edu/CaltechAUTHORS:20140318-101020463

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Abstract

Chlamydiae comprise important pathogenic and symbiotic bacteria that alternate between morphologically and physiologically different life stages during their developmental cycle. Using electron cryotomography, we characterize the ultrastructure of the developmental stages of three environmental chlamydiae: Parachlamydia acanthamoebae, Protochlamydia amoebophila and Simkania negevensis. We show that chemical fixation and dehydration alter the cell shape of Parachlamydia and that the crescent body is not a developmental stage, but an artefact of conventional electron microscopy. We further reveal type III secretion systems of environmental chlamydiae at macromolecular resolution and find support for a chlamydial needle-tip protein. Imaging bacteria inside their host cells by cryotomography for the first time, we observe marked differences in inclusion morphology and development as well as host organelle recruitment between the three chlamydial organisms, with Simkania inclusions being tightly enveloped by the host endoplasmic reticulum. The study demonstrates the power of electron cryotomography to reveal structural details of bacteria–host interactions that are not accessible using traditional methods.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1111/1462-2920.12299DOIArticle
http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12299/abstractPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4949044PubMed CentralArticle
ORCID:
AuthorORCID
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2013 Society for Applied Microbiology and John Wiley & Sons Ltd. Received 8 August, 2013; accepted 26 September, 2013. Article first published online: 8 Nov 2013. This work was funded by the Austrian Science Fund FWF (Y277-B03 to MH), the European Research Council (ERC StG ‘EvoChlamy’ to MH), the Caltech Center for Environmental Microbiology Interactions (to GJJ, MP), and a gift from the Gordon and Betty Moore Foundation to Caltech.
Group:Caltech Center for Environmental Microbial Interactions (CEMI)
Funders:
Funding AgencyGrant Number
Austrian Science Fund (FWF)Y277-B03
European Research Council (ERC StG EvoChlamy)UNSPECIFIED
Caltech Center for Environmental Microbial Interactions (CEMI)UNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
PubMed Central ID:PMC4949044
Record Number:CaltechAUTHORS:20140318-101020463
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20140318-101020463
Official Citation:Pilhofer, M., Aistleitner, K., Ladinsky, M. S., König, L., Horn, M. and Jensen, G. J. (2014), Architecture and host interface of environmental chlamydiae revealed by electron cryotomography. Environmental Microbiology, 16: 417–429. doi: 10.1111/1462-2920.12299
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:44375
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:18 Mar 2014 17:37
Last Modified:20 Oct 2017 21:28

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