Yuet, Kai P. and Tirrell, David A. (2014) Chemical Tools for Temporally and Spatially Resolved Mass Spectrometry-Based Proteomics. Annals of Biomedical Engineering, 42 (2). pp. 299-311. ISSN 0090-6964. PMCID PMC3925203. https://resolver.caltech.edu/CaltechAUTHORS:20140404-094604296
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Abstract
Accurate measurements of the abundances, synthesis rates and degradation rates of cellular proteins are critical for understanding how cells and organisms respond to changes in their environments. Over the past two decades, there has been increasing interest in the use of mass spectrometry for proteomic analysis. In many systems, however, protein diversity as well as cell and tissue heterogeneity limit the usefulness of mass spectrometry-based proteomics. As a result, researchers have had difficulty in systematically identifying proteins expressed within specified time intervals, or low abundance proteins expressed in specific tissues or in a few cells in complex microbial systems. In this review, we present recently-developed tools and strategies that probe these two subsets of the proteome: proteins synthesized during well-defined time intervals—temporally resolved proteomics—and proteins expressed in predetermined cell types, cells or cellular compartments—spatially resolved proteomics—with a focus on chemical and biological mass spectrometry-based methodologies.
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Additional Information: | © 2013 Biomedical Engineering Society. Received 11 July 2013; accepted 24 July 2013; published online 14 August 2013. Work at Caltech on non-canonical amino acid tagging is supported by National Institutes of Health grant NIH R01 GM062523 and by the Institute for Collaborative Biotechnologies through grant W911NF-09-0001 from U.S. Army Research Office. | ||||||||||||
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Subject Keywords: | Protein synthesis; BONCAT; SILAC; APEX | ||||||||||||
Issue or Number: | 2 | ||||||||||||
PubMed Central ID: | PMC3925203 | ||||||||||||
Record Number: | CaltechAUTHORS:20140404-094604296 | ||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20140404-094604296 | ||||||||||||
Official Citation: | Yuet, K., & Tirrell, D. (2014). Chemical Tools for Temporally and Spatially Resolved Mass Spectrometry-Based Proteomics. Annals of Biomedical Engineering, 42(2), 299-311. doi: 10.1007/s10439-013-0878-3 | ||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
ID Code: | 44658 | ||||||||||||
Collection: | CaltechAUTHORS | ||||||||||||
Deposited By: | Tony Diaz | ||||||||||||
Deposited On: | 07 Apr 2014 17:30 | ||||||||||||
Last Modified: | 21 Apr 2020 22:01 |
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