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Chemical Tools for Temporally and Spatially Resolved Mass Spectrometry-Based Proteomics

Yuet, Kai P. and Tirrell, David A. (2014) Chemical Tools for Temporally and Spatially Resolved Mass Spectrometry-Based Proteomics. Annals of Biomedical Engineering, 42 (2). pp. 299-311. ISSN 0090-6964. PMCID PMC3925203. doi:10.1007/s10439-013-0878-3.

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Accurate measurements of the abundances, synthesis rates and degradation rates of cellular proteins are critical for understanding how cells and organisms respond to changes in their environments. Over the past two decades, there has been increasing interest in the use of mass spectrometry for proteomic analysis. In many systems, however, protein diversity as well as cell and tissue heterogeneity limit the usefulness of mass spectrometry-based proteomics. As a result, researchers have had difficulty in systematically identifying proteins expressed within specified time intervals, or low abundance proteins expressed in specific tissues or in a few cells in complex microbial systems. In this review, we present recently-developed tools and strategies that probe these two subsets of the proteome: proteins synthesized during well-defined time intervals—temporally resolved proteomics—and proteins expressed in predetermined cell types, cells or cellular compartments—spatially resolved proteomics—with a focus on chemical and biological mass spectrometry-based methodologies.

Item Type:Article
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URLURL TypeDescription CentralArticle ReadCube access
Yuet, Kai P.0000-0002-1381-8923
Tirrell, David A.0000-0003-3175-4596
Other Contributors:
ContributionOther Contributors NameIdentifierPersonID (may be blank)
EditorNerem, RobertNerem-RUNSPECIFIED
Additional Information:© 2013 Biomedical Engineering Society. Received 11 July 2013; accepted 24 July 2013; published online 14 August 2013. Work at Caltech on non-canonical amino acid tagging is supported by National Institutes of Health grant NIH R01 GM062523 and by the Institute for Collaborative Biotechnologies through grant W911NF-09-0001 from U.S. Army Research Office.
Funding AgencyGrant Number
NIHR01 GM062523
Army Research Office (ARO)W911NF-09-0001
Subject Keywords:Protein synthesis; BONCAT; SILAC; APEX
Issue or Number:2
PubMed Central ID:PMC3925203
Record Number:CaltechAUTHORS:20140404-094604296
Persistent URL:
Official Citation:Yuet, K., & Tirrell, D. (2014). Chemical Tools for Temporally and Spatially Resolved Mass Spectrometry-Based Proteomics. Annals of Biomedical Engineering, 42(2), 299-311. doi: 10.1007/s10439-013-0878-3
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:44658
Deposited By: Tony Diaz
Deposited On:07 Apr 2014 17:30
Last Modified:10 Nov 2021 16:54

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