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Carbon nanotube-based substrates for modulation of human pluripotent stem cell fate

Pryzhkova, Marina V. and Aria, Indrat and Cheng, Qingsu and Harris, Greg M. and Zang, Xingjie and Gharib, Morteza and Jabbarzadeh, Ehsan (2014) Carbon nanotube-based substrates for modulation of human pluripotent stem cell fate. Biomaterials, 35 (19). pp. 5098-5109. ISSN 0142-9612. PMCID PMC4943838. http://resolver.caltech.edu/CaltechAUTHORS:20140408-090530812

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Abstract

We investigated the biological response of human pluripotent stem cells (hPSCs) cultured on a carbon nanotube (CNT) array-based substrate with the long term goal to direct hPSC germ layer specification for a wide variety of tissue engineering applications. CNT arrays were fabricated using a chemical vapor deposition system allowing for control over surface roughness and mechanical stiffness. Our results demonstrated that hPSCs readily attach to hydrophilized and extracellular matrix coated CNT arrays. hPSCs cultured as colonies in conditions supporting self-renewal demonstrated the morphology and marker expression of undifferentiated hPSCs. Conditions inducing spontaneous differentiation lead to hPSC commitment to all three embryonic germ layers as assessed by immunostaining and RT-PCR analysis. Strikingly, the physical characteristics of CNT arrays favored mesodermal specification of hPSCs. This is contradictory to the behavior of hPSCs on traditional tissue culture plastic which promotes the development of ectoderm. Altogether, these results demonstrate the potential of CNT arrays to be used in the generation of new platforms that allow for precise control of hPSC differentiation by tuning the characteristics of their physical microenvironment.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.biomaterials.2014.03.011DOIArticle
http://www.sciencedirect.com/science/article/pii/S0142961214002464PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4943838PubMed CentralArticle
ORCID:
AuthorORCID
Gharib, Morteza0000-0002-2204-9302
Additional Information:© 2014 Elsevier Ltd. Received 13 January 2014; Accepted 7 March 2014; Available online 29 March 2014. We wish to thank Dr. I. Slukvin and Dr. K. Hu (University of Wisconsin-Madison) for hiPSC lines provided. We also would like to acknowledge Dr. Julia Greer’s help with nanoindentation measurements of carbon nanotube arrays. We also would like to acknowledge W. M. Keck Foundation Open Laboratory at USC for the help with AFM measurements. We gratefully acknowledge support from the National Institute of Health (Grant NIH P20 GM103641) and National Science Foundation (Grant EPS-0903795).
Group:GALCIT
Funders:
Funding AgencyGrant Number
W. M. Keck FoundationUNSPECIFIED
NIHP20 GM103641
NSFEPS-0903795
PubMed Central ID:PMC4943838
Record Number:CaltechAUTHORS:20140408-090530812
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20140408-090530812
Official Citation:Marina V. Pryzhkova, Indrat Aria, Qingsu Cheng, Greg M. Harris, Xingjie Zan, Morteza Gharib, Ehsan Jabbarzadeh, Carbon nanotube-based substrates for modulation of human pluripotent stem cell fate, Biomaterials, Volume 35, Issue 19, June 2014, Pages 5098-5109, ISSN 0142-9612, http://dx.doi.org/10.1016/j.biomaterials.2014.03.011. (http://www.sciencedirect.com/science/article/pii/S0142961214002464)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:44732
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:08 Apr 2014 16:44
Last Modified:19 Jul 2017 21:55

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