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Delayed and isoform-specific effect of NMDA exposure on neural cell adhesion molecules in hippocampus

Hoffman, Keith B. and Murray, Ben A. and Lynch, Gary and Munirathinam, Subramani and Bahr, Ben A. (2001) Delayed and isoform-specific effect of NMDA exposure on neural cell adhesion molecules in hippocampus. Neuroscience Research, 39 (2). pp. 167-173. ISSN 0168-0102. doi:10.1016/S0168-0102(00)00214-5.

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Brief stimulation of N-methyl-d-aspartate (NMDA) receptors has been shown to generate proteolytic fragments from the extracellular domain of neural cell adhesion molecules (NCAMs). In the present study, hippocampal slice cultures were used to demonstrate that such brief stimulation is followed by a delayed increase in the 180-kDa isoform NCAM-180. The slices were exposed to NMDA for 30 s followed by rapid quenching with the antagonist AP5. Immunoassays of the experimental samples indicated that concentrations of NCAM-180 were elevated above matched controls 2–3 h after the NMDA exposure, but not at earlier or later time points. This effect was isoform-specific as concentrations of the 140-kDa NCAM species were not found to increase. Interestingly, similar selectivity was evident with prolonged infusions of NMDA where, in contrast to the effect of brief stimulation, NCAM-180 content was reduced to 50% while levels of NCAM-140 were unchanged. Together with previous findings, the data indicate that the synaptic chemistries activated by NMDA differentially regulate NCAM-180 at the translation level and by localized activation of proteases.

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Additional Information:© 2001 Elsevier Science Ireland Ltd and the Japan Neuroscience Society. Received 7 February 2000; accepted 2 October 2000. The authors thank Drs M. Kessler and J. Larson for helpful advice and Ms Alyson M. Blow for excellent editorial services. This work was supported by NSF grant IBN-9220156 to B.A.M., AFOSR grant 95-I0304 to G.L., and U.S. Army Medical Research grant DAMD17-99-C9090 to B.A.B.
Funding AgencyGrant Number
Air Force Office of Scientific Research (AFOSR)95-I0304
U. S. Army Medical Research grantDAMD17-99-C9090
Subject Keywords:NCAMs; NMDA receptors; Proteolysis; Synaptic adhesion; Long-term potentiation
Issue or Number:2
Record Number:CaltechAUTHORS:20140411-150023278
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Official Citation:Keith B. Hoffman, Ben A. Murray, Gary Lynch, Subramani Munirathinam, Ben A. Bahr, Delayed and isoform-specific effect of NMDA exposure on neural cell adhesion molecules in hippocampus, Neuroscience Research, Volume 39, Issue 2, February 2001, Pages 167-173, ISSN 0168-0102, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:44896
Deposited By: Ruth Sustaita
Deposited On:11 Apr 2014 22:30
Last Modified:10 Nov 2021 16:57

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