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Using electroporation and lipid-mediated transfection of GFP-expressing plasmids to label embryonic avian cells for vital confocal and two-photon microscopy

Dickinson, Mary E. and Murray, Ben A. and Haynes, Sherry M. and Waters, Christopher W. and Longmuir, Kenneth J. (2002) Using electroporation and lipid-mediated transfection of GFP-expressing plasmids to label embryonic avian cells for vital confocal and two-photon microscopy. Differentiation, 70 (4-5). pp. 172-180. ISSN 0301-4681. https://resolver.caltech.edu/CaltechAUTHORS:20140414-115059414

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Abstract

Fluorescent proteins have emerged as an ideal fluorescent marker for studying cell morphologies in vital systems. These proteins were first applied in whole organisms with established germ-line transformation protocols, but now it is possible to label cells with fluorescent proteins in other organisms. Here we present two ways to introduce GFP expressing plasmids into avian embryos for vital confocal and two-photon imaging. First, electroporation is a powerful approach to introduce GFP into the developing neural tube, offering several advantages over dye labeling. Second, we introduce a new lipid-based transfection system for introducing plasmid DNA directly to a small group of injected cells within live, whole embryos. These complementary approaches make it possible to transfect a wide-range of cell types in the avian embryo and the bright, stable, uniform expression of GFP offers great advantages for vital fluorescence imaging.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1046/j.1432-0436.2002.700407.xDOIArticle
http://www.sciencedirect.com/science/article/pii/S0301468109604473PublisherArticle
Additional Information:© 2002 Blackwell Verlag. Accepted in revised form: 17 April 2002.
Subject Keywords:GFP; fluorescence; confocal and two-photon microscopy; embryo; lipofection; electroporation
Issue or Number:4-5
Record Number:CaltechAUTHORS:20140414-115059414
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20140414-115059414
Official Citation:Mary E. Dickinson, Ben A. Murray, Christopher W. Waters, Sherry M Haynes, Kenneth J. Longmuir, Using electroporation and lipid-mediated transfection of GFP-expressing plasmids to label embryonic avian cells for vital confocal and two-photon microscopy, Differentiation, Volume 70, Issues 4–5, June 2002, Pages 172-180, ISSN 0301-4681, http://dx.doi.org/10.1046/j.1432-0436.2002.700407.x. (http://www.sciencedirect.com/science/article/pii/S0301468109604473)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:44927
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Apr 2014 20:24
Last Modified:03 Oct 2019 06:24

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