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Isobutanol production at elevated temperatures in thermophilic Geobacillus thermoglucosidasius

Lin, Paul P. and Rabe, Kersten S. and Takasumi, Jennifer L. and Kadisch, Marvin and Arnold, Frances H. and Liao, James C. (2014) Isobutanol production at elevated temperatures in thermophilic Geobacillus thermoglucosidasius. Metabolic Engineering, 24 . pp. 1-8. ISSN 1096-7176. doi:10.1016/j.ymben.2014.03.006.

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The potential advantages of biological production of chemicals or fuels from biomass at high temperatures include reduced enzyme loading for cellulose degradation, decreased chance of contamination, and lower product separation cost. In general, high temperature production of compounds that are not native to the thermophilic hosts is limited by enzyme stability and the lack of suitable expression systems. Further complications can arise when the pathway includes a volatile intermediate. Here we report the engineering of Geobacillus thermoglucosidasius to produce isobutanol at 50 °C. We prospected various enzymes in the isobutanol synthesis pathway and characterized their thermostabilities. We also constructed an expression system based on the lactate dehydrogenase promoter from Geobacillus thermodenitrificans. With the best enzyme combination and the expression system, 3.3 g/l of isobutanol was produced from glucose and 0.6 g/l of isobutanol from cellobiose in G. thermoglucosidasius within 48 h at 50 °C. This is the first demonstration of isobutanol production in recombinant bacteria at an elevated temperature.

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Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2014 International Metabolic Engineering Society. Published by Elsevier Inc. Received 1 March 2014; Accepted 31 March 2014. Available online 8 April 2014. Research at UCLA was supported by National Science Foundation award (MCB-1139318) and DOE BioEnergy Science Center (BESC). Research at Caltech was funded by the Division of Molecular and Cellular Biosciences through grant MCB-0903817 from the National Science Foundation. This material is based upon research performed in a renovated collaborator by the National Science Foundation under Grant No. 0963183, which is an award funded under the American Recovery and Reinvestment Act of 2009 (ARRA). K.S.R. thanks the Deutscher Akademischer Austauschdienst (DAAD) for a postdoctoral fellowship. We thank Hidevaldo B. Machado, Kouki M. Yoshino, Jing-Yao Chen and Christopher Snow for their technical assistance.
Funding AgencyGrant Number
Department of Energy (DOE)UNSPECIFIED
American Recovery and Reinvestment Act (ARRA)UNSPECIFIED
Deutscher Akademischer Austauschdienst (DAAD)UNSPECIFIED
Subject Keywords:Biofuel; Metabolic engineering; Thermophile; Butanol
Record Number:CaltechAUTHORS:20140421-110219081
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Official Citation:Paul P. Lin, Kersten S. Rabe, Jennifer L. Takasumi, Marvin Kadisch, Frances H. Arnold, James C. Liao, Isobutanol production at elevated temperatures in thermophilic Geobacillus thermoglucosidasius, Metabolic Engineering, Volume 24, July 2014, Pages 1-8, ISSN 1096-7176, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:45074
Deposited By: Tony Diaz
Deposited On:21 Apr 2014 22:20
Last Modified:10 Nov 2021 17:00

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