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Protein Nanoparticles Engineered to Sense Kinase Activity in MRI

Shapiro, Mikhail G. and Szablowski, Jerzy O. and Langer, Robert and Jasanoff, Alan (2009) Protein Nanoparticles Engineered to Sense Kinase Activity in MRI. Journal of the American Chemical Society, 131 (7). pp. 2484-2486. ISSN 0002-7863. PMCID PMC2662502. https://resolver.caltech.edu/CaltechAUTHORS:20140423-132454335

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Abstract

We introduce a family of protein nanoparticles capable of sensing analytes in conjunction with magnetic resonance imaging (MRI). The new sensors are derived from the iron storage protein ferritin (Ft); they are designed and optimized using facile protein engineering methods, and self-assembled in cells harboring specific combinations of DNA coding sequences. As illustration, we show that suitably constructed Ft-based sensors can report activity of the important neural signaling enzyme protein kinase A (PKA). Phosphorylation of the engineered Ft-based nanoparticles by PKA promotes clustering and changes in T_2-weighted MRI signal.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja8086938 DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja8086938PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja8086938PublisherSupporting Information
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2662502/PubMed CentralArticle
ORCID:
AuthorORCID
Shapiro, Mikhail G.0000-0002-0291-4215
Szablowski, Jerzy O.0000-0001-7851-5408
Jasanoff, Alan0000-0002-2834-6359
Additional Information:© 2009 American Chemical Society. Received November 12, 2008. Publication Date (Web): January 29, 2009. The authors thank Michael Greenberg for supplying clones for CREB and CBP, and thank Paolo Arioso for providing the human HF and LF genes. The authors are also grateful to Niyatee Samudra for assistance with DNA cloning and Vibhu Sachdev for help obtaining TEM images. M.G.S. wishes to acknowledge the Fannie and John Hertz Foundation and the Paul and Daisy Soros Fellowship for generous support. This work was funded in part by a New Innovator Award NIH DP2-OD2441 to A.J., and NIH grant number R01-DE013023- 08 to R.L.
Funders:
Funding AgencyGrant Number
Fannie and John Hertz FoundationUNSPECIFIED
Paul and Daisy Soros FellowshipUNSPECIFIED
NIHDP2-OD2441
NIHR01-DE013023-08
Issue or Number:7
PubMed Central ID:PMC2662502
Record Number:CaltechAUTHORS:20140423-132454335
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20140423-132454335
Official Citation:Protein Nanoparticles Engineered to Sense Kinase Activity in MRI Mikhail G. Shapiro, Jerzy O. Szablowski, Robert Langer, and Alan Jasanoff Journal of the American Chemical Society 2009 131 (7), 2484-2486
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:45149
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Apr 2014 20:44
Last Modified:09 Mar 2020 13:18

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