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The Thicknesses of Hemoglobin and Bovine Serum Albumin Molecules as Unimolecular Layers Adsorbed onto Films of Barium Stearate

Fisk, Albert A. (1950) The Thicknesses of Hemoglobin and Bovine Serum Albumin Molecules as Unimolecular Layers Adsorbed onto Films of Barium Stearate. Proceedings of the National Academy of Sciences of the United States of America, 36 (10). pp. 518-523. ISSN 0027-8424.

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The following work, which describes a method of measuring one dimension of some protein molecules, is based on the determination of the apparent thickness of a unimolecular layer of globular protein molecules adsorbed from solution onto a metallic slide covered with an optical gauge of barium stearate. Langmuir [1, 2] and Rothen [3] have published a few results obtained by such a technique, but have not exploited the method thoroughly. A complete set of experimental data has been obtained by Clowes [4] on insulin and protamine. He studied the effects of pH and time of exposure on the thickness of layers of protamine and insulin adsorbed onto slides covered with barium stearate and conditioned with uranyl acetate. He found that the pH was responsible for large variations in the thickness of the adsorbed layers and that the thickness of insulin layers adsorbed onto a protamine base was dependent on the concentration of the insulin. Since Clowes found thicknesses as high as 100 Å. for protamine and 400 Å. for insulin, he was without doubt usually dealing with multilayers.

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Additional Information:Copyright © 1950 by the National Academy of Sciences Communicated by Linus Pauling, July 17, 1950 This work was supported in part by grants from The American Cancer Society and the U. S. Public Health Service. [A.A.F. was an] American Cancer Society Fellow. Gates and Crellin Laboratories of Chemistry, California Institute of Technology, Pasandea, California Contribution No. 1422.
Issue or Number:10
Record Number:CaltechAUTHORS:FISpnas50
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4531
Deposited By: Archive Administrator
Deposited On:27 Aug 2006
Last Modified:02 Oct 2019 23:13

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