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Directing Neuronal Signaling through Cell-Surface Glycan Engineering

Pulsipher, Abigail and Griffin, Matthew E. and Stone, Shannon E. and Brown, Joshua M. and Hsieh-Wilson, Linda C. (2014) Directing Neuronal Signaling through Cell-Surface Glycan Engineering. Journal of the American Chemical Society, 136 (19). pp. 6794-6797. ISSN 0002-7863. PMCID PMC4120997. http://resolver.caltech.edu/CaltechAUTHORS:20140505-091717887

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Abstract

The ability to tailor plasma membranes with specific glycans may enable the control of signaling events that are critical for proper development and function. We report a method to modify cell surfaces with specific sulfated chondroitin sulfate (CS) glycosaminoglycans using chemically modified liposomes. Neurons engineered to display CS-E-enriched polysaccharides exhibited increased activation of neurotrophin-mediated signaling pathways and enhanced axonal growth. This approach provides a facile, general route to tailor cell membranes with biologically active glycans and demonstrates the potential to direct important cellular events through cell-surface glycan engineering.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja5005174DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja5005174PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja5005174PublisherSupporting Information
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4120997/PubMed CentralArticle
ORCID:
AuthorORCID
Stone, Shannon E.0000-0002-6617-3874
Hsieh-Wilson, Linda C.0000-0001-5661-1714
Additional Information:© 2014 American Chemical Society. ACS AuthorChoice. Received: January 17, 2014; publication Date (Web): April 18, 2014. We thank Rochelle Diamond at the Caltech Flow Cytometry Facility for flow cytometry expertise, Carol Garland for TEM imaging, and Jennifer Keeffe and Dorothy Pan for DLS and zeta potential analyses aid, respectively. This work was supported by a grant from the NIH (R01 GM093627) and an NSF GRF (M.E.G., DGE-1144469).
Funders:
Funding AgencyGrant Number
NIHR01 GM093627
NSF Graduate Research FellowshipDGE-1144469
PubMed Central ID:PMC4120997
Record Number:CaltechAUTHORS:20140505-091717887
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20140505-091717887
Official Citation:Directing Neuronal Signaling through Cell-Surface Glycan Engineering Abigail Pulsipher, Matthew E. Griffin, Shannon E. Stone, Joshua M. Brown, and Linda C. Hsieh-Wilson Journal of the American Chemical Society 2014 136 (19), 6794-6797
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:45478
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:05 May 2014 19:53
Last Modified:06 Nov 2017 21:47

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