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Developmental Self-Assembly of a DNA Tetrahedron

Sadowski, John P. and Calvert, Colby R. and Zhang, David Yu and Pierce, Niles A. and Yin, Peng (2014) Developmental Self-Assembly of a DNA Tetrahedron. ACS Nano, 8 (4). pp. 3251-3259. ISSN 1936-0851 . PMCID PMC4004324. http://resolver.caltech.edu/CaltechAUTHORS:20140603-072449619

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Abstract

Kinetically controlled isothermal growth is fundamental to biological development, yet it remains challenging to rationally design molecular systems that self-assemble isothermally into complex geometries via prescribed assembly and disassembly pathways. By exploiting the programmable chemistry of base pairing, sophisticated spatial and temporal control have been demonstrated in DNA self-assembly, but largely as separate pursuits. By integrating temporal with spatial control, here we demonstrate the “developmental” self-assembly of a DNA tetrahedron, where a prescriptive molecular program orchestrates the kinetic pathways by which DNA molecules isothermally self-assemble into a well-defined three-dimensional wireframe geometry. In this reaction, nine DNA reactants initially coexist metastably, but upon catalysis by a DNA initiator molecule, navigate 24 individually characterizable intermediate states via prescribed assembly pathways, organized both in series and in parallel, to arrive at the tetrahedral final product. In contrast to previous work on dynamic DNA nanotechnology, this developmental program coordinates growth of ringed substructures into a three-dimensional wireframe superstructure, taking a step toward the goal of kinetically controlled isothermal growth of complex three-dimensional geometries.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/nn4038223DOIArticle
http://pubs.acs.org/doi/abs/10.1021/nn4038223PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/nn4038223PublisherSupporting Information
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4004324/PubMed CentralArticle
ORCID:
AuthorORCID
Pierce, Niles A.0000-0003-2367-4406
Additional Information:© 2014 American Chemical Society. ACS AuthorChoice - This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. Received for review July 23, 2013 and accepted February 19, 2014. Published online April 11, 2014. The authors thank C. Grun and E. Winfree for discussions, and D. Pastuszak for help with draft preparation. This work was funded by Office of Naval Research Grants N000141010827 and N000141310593, Office of Naval Research Young Investigator Program Award N000141110914, NIH Director's New Innovator Award 1DP2OD007292, NSF CAREER Award CCF1054898, NSF Grant CCF1162459, NSF Expedition in Computing Award CCF1317291, and Wyss Institute for Biologically Inspired Engineering Faculty Startup Fund to P.Y., and NIH 5R01CA140759, NIH P50 HG004071, the Molecular Programming Project (NSF-CCF-0832824 and NSF-CCF-1317694), and the Gordon and Betty Moore Foundation (GBMF2809) to N.A.P.; J.P.S. acknowledges a Graduate Research Fellowship from NSF and a J. Marshall & Jane H. Booker Graduate Scholarship from the Buttonwood Foundation, and D.Y.Z. is supported by NIH Transition to Independence Award 1K99EB015331.
Funders:
Funding AgencyGrant Number
Office of Naval Research (ONR)N000141010827
Office of Naval Research (ONR)N000141310593
Office of Naval Research (ONR)N000141110914
NIH1DP2OD007292
NSFCCF-1054898
NSFCCF-1162459
NSFCCF-1317291
Wyss Institute for Biologically Inspired Engineering Faculty Startup FundUNSPECIFIED
NIH5R01CA140759
NIHP50 HG004071
NSFCCF-0832824
NSFCCF-1317694
Gordon and Betty Moore FoundationGBMF2809
NSF Graduate Research FellowshipUNSPECIFIED
Buttonwood FoundationUNSPECIFIED
NIH1K99EB015331
Subject Keywords:molecular programming; DNA nanotechnology; kinetic pathway control; developmental self-assembly
PubMed Central ID:PMC4004324
Record Number:CaltechAUTHORS:20140603-072449619
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20140603-072449619
Official Citation:Developmental Self-Assembly of a DNA Tetrahedron John P. Sadowski, Colby R. Calvert, David Yu Zhang, Niles A. Pierce, and Peng Yin ACS Nano 2014 8 (4), 3251-3259
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:46037
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:03 Jun 2014 15:17
Last Modified:21 Jul 2017 16:25

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