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A Toolbox of Aedes Aegypti Germline Regulatory Elements for the Development of Genetic Drive Systems and Population Control

Papathanos, Philippos A. and Akbari, Omar S. and Hay, Bruce A. (2013) A Toolbox of Aedes Aegypti Germline Regulatory Elements for the Development of Genetic Drive Systems and Population Control. Pathogens and Global Health, 107 (8). p. 445. ISSN 2047-7724. doi:10.1179/2047772413Z.000000000160. https://resolver.caltech.edu/CaltechAUTHORS:20140603-135424481

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Abstract

Experiments in Drosophila have demonstrated that Medea-like genetic drive systems are engineer-able and can permit invasion of synthetic transgenes in wild populations. Similar accomplishments have also been made in Anopheles strains using engineered HEG-based gene drive systems. Synthetic circuits that generate a novel selfish phenotype typically require efficient germline expression of transgenes. To enable the transfer of this technology to Aedes mosquitoes, we have characterized a repertoire of promoters for expression of transgenes in germline tissues. Candidates were selected on the basis of expression data from a NGS transcriptome study and on evolutionary relationships to known maternal genes. The expression of eGFP driven by five Aedes aegypti and one known Anopheles gambiae germline specific regulatory elements was analyzed in transgenic reporter strains. Overall, the tools represent a significant expansion of regulatory elements for germline manipulation in this mosquito. I will describe the assorted expression profiles and discuss possible downstream applications.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.maneyonline.com/doi/abs/10.1179/2047772413Z.000000000160PublisherConference Proceedings
http://dx.doi.org/10.1179/2047772413Z.000000000160DOIConference Proceedings
ORCID:
AuthorORCID
Akbari, Omar S.0000-0002-6853-9884
Additional Information:© 2013 Maney Publishing.
Issue or Number:8
DOI:10.1179/2047772413Z.000000000160
Record Number:CaltechAUTHORS:20140603-135424481
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20140603-135424481
Official Citation:"EMBO Conference on Molecular and Population Biology of Mosquitoes and Other Disease Vectors: From Basic Vector Biology to Disease Control." Pathogens and Global Health, 107(8), pp. 400–457
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:46052
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:03 Jun 2014 21:04
Last Modified:10 Nov 2021 17:20

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