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Axial standing-wave illumination frequency-domain imaging (SWIF)

Judkewitz, Benjamin and Yang, Changhuei (2014) Axial standing-wave illumination frequency-domain imaging (SWIF). Optics Express, 22 (9). pp. 11001-11010. ISSN 1094-4087. PMCID PMC4083045. doi:10.1364/OE.22.011001.

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Despite their tremendous contribution to biomedical research and diagnosis, conventional spatial sampling techniques such as wide-field, point scanning or selective plane illumination microscopy face inherent limiting trade-offs between spatial resolution, field-of-view, phototoxicity and recording speed. Several of these trade-offs are the result of spatial sampling with diffracting beams. Here, we introduce a new strategy for fluorescence imaging, SWIF, which instead encodes the axial profile of a sample in the Fourier domain. We demonstrate how this can be achieved with propagation-invariant illumination patterns that extend over several millimeters and robustly propagate through layers of varying refractive index. This enabled us to image a lateral field-of-view of 0.8 mm x 1.5 mm with an axial resolution of 2.4 µm – greatly exceeding the lateral field-of-view of conventional illumination techniques (~100 µm) at comparable resolution. Thus, SWIF allowed us to surpass the limitations of diffracting illumination beams and untangle lateral field-of-view from resolution.

Item Type:Article
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URLURL TypeDescription DOIArticle CentralArticle
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2014 Optical Society of America. Received 21 Mar 2014; revised 22 Apr 2014; accepted 23 Apr 2014; published 30 Apr 2014. We thank Ying-Min Wang, Roarke Horstmeyer and Phil Willems for valuable advice and for critically reading the manuscript. This work was supported by the National Institutes of Health (1DP2OD007307-01). Benjamin Judkewitz is a recipient of the Sir Henry Wellcome Postdoctoral Fellowship by the Wellcome Trust.
Funding AgencyGrant Number
Sir Henry Wellcome FellowshipUNSPECIFIED
Issue or Number:9
Classification Code:OCIS codes: (110.0180) Microscopy; (110.2945) Illumination design; (110.6880) Three-dimensional image acquisition
PubMed Central ID:PMC4083045
Record Number:CaltechAUTHORS:20140605-130038426
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:46100
Deposited By: Tony Diaz
Deposited On:05 Jun 2014 21:01
Last Modified:10 Nov 2021 17:20

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