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Microfluidics-Based Single-Cell Functional Proteomics for Fundamental and Applied Biomedical Applications

Yu, Jing and Zhou, Jing and Sutherland, Alex and Wei, Wei and Shin, Young Shik and Xue, Min and Heath, James R. (2014) Microfluidics-Based Single-Cell Functional Proteomics for Fundamental and Applied Biomedical Applications. Annual Review of Analytical Chemistry, 7 . pp. 275-295. ISSN 1936-1327. http://resolver.caltech.edu/CaltechAUTHORS:20140609-131536570

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Abstract

We review an emerging microfluidics-based toolkit for single-cell functional proteomics. Functional proteins include, but are not limited to, the secreted signaling proteins that can reflect the biological behaviors of immune cells or the intracellular phosphoproteins associated with growth factor–stimulated signaling networks. Advantages of the microfluidics platforms are multiple. First, 20 or more functional proteins may be assayed simultaneously from statistical numbers of single cells. Second, cell behaviors (e.g., motility) may be correlated with protein assays. Third, extensions to quantized cell populations can permit measurements of cell–cell interactions. Fourth, rare cells can be functionally identified and then separated for further analysis or culturing. Finally, certain assay types can provide a conduit between biology and the physicochemical laws. We discuss the history and challenges of the field then review design concepts and uses of the microchip platforms that have been reported, with an eye toward biomedical applications. We then look to the future of the field.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1146/annurev-anchem-071213-020323DOIArticle
http://www.annualreviews.org/doi/abs/10.1146/annurev-anchem-071213-020323PublisherArticle
ORCID:
AuthorORCID
Heath, James R.0000-0001-5356-4385
Additional Information:© 2014 Annual Reviews. First published online as a Review in Advance on June 2, 2014. A portion of the science described in this review was funded by the National Cancer Institute grants 5U54CA119347 and R01 CA170689-01, the Jean Perkins Foundation, and the Ben and Catherine Ivy Foundation. The authors are not aware of any affiliations, memberships, funding, or financial holdings that might be perceived as affecting the objectivity of this review.
Funders:
Funding AgencyGrant Number
National Cancer Institute5U54CA119347
National Cancer InstituteR01 CA170689-01
Jean Perkins FoundationUNSPECIFIED
Ben and Catherine Ivy FoundationUNSPECIFIED
Subject Keywords:single-cell proteomics, microfluidics, immunoassays, protein signaling
Record Number:CaltechAUTHORS:20140609-131536570
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20140609-131536570
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:46156
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:09 Jun 2014 21:31
Last Modified:06 Mar 2015 18:14

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