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The Stem Cell Niche in Leaf Axils Is Established by Auxin and Cytokinin in Arabidopsis

Wang, Ying and Wang, Jin and Shi, Bihai and Yu, Ting and Qi, Jiyan and Meyerowitz, Elliot M. and Jiao, Yuling (2014) The Stem Cell Niche in Leaf Axils Is Established by Auxin and Cytokinin in Arabidopsis. Plant Cell, 26 (5). pp. 2055-2067. ISSN 1040-4651. PMCID PMC4079368. doi:10.1105/tpc.114.123083.

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Plants differ from most animals in their ability to initiate new cycles of growth and development, which relies on the establishment and activity of branch meristems harboring new stem cell niches. In seed plants, this is achieved by axillary meristems, which are established in the axil of each leaf base and develop into lateral branches. Here, we describe the initial processes of Arabidopsis thaliana axillary meristem initiation. Using reporter gene expression analysis, we find that axillary meristems initiate from leaf axil cells with low auxin through stereotypical stages. Consistent with this, ectopic overproduction of auxin in the leaf axil efficiently inhibits axillary meristem initiation. Furthermore, our results demonstrate that auxin efflux is required for the leaf axil auxin minimum and axillary meristem initiation. After lowering of auxin levels, a subsequent cytokinin signaling pulse is observed prior to axillary meristem initiation. Genetic analysis suggests that cytokinin perception and signaling are both required for axillary meristem initiation. Finally, we show that cytokinin overproduction in the leaf axil partially rescue axillary meristem initiation-deficient mutants. These results define a mechanistic framework for understanding axillary meristem initiation.

Item Type:Article
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URLURL TypeDescription Figures and Tables CentralArticle
Wang, Jin0000-0002-4924-1716
Meyerowitz, Elliot M.0000-0003-4798-5153
Jiao, Yuling0000-0002-1189-1676
Additional Information:© 2014 American Society of Plant Biologists. Received January 16, 2014. Revised April 18, 2014. Accepted April 29, 2014; published May 21, 2014. Accession Numbers Sequence data from this article can be found in the Arabidopsis Genome initiative or GenBank/EMBL databases under the following accession numbers: At5g35750 (AHK2), At1g27320 (AHK3), At2g01830 (AHK4), At3g16857 (ARR1), At4g31920 (ARR10), At1g67710 (ARR11), At2g25180 (ARR12), At5g53950 (CUC2), At3g19160 (IPT8), At1g55580 (LAS), At2g34650 (PID), At1g73590 (PIN1), At5g23000 (RAX1), At2g36890 (RAX2), At3g49690 (RAX3), At1g62360 (STM), and AB025110 (iaaM). We thank M. Aida, J.L. Celenza, Y. Eshed, T. Kakimoto, M. Tasaka, K. Theres, M. Tsiantis, C. Ueguchi, J. Zuo, and the ABRC for seeds and plasmids and C. Li for allowing us to use his tomato growth facility. We also thank J. Zuo and two anonymous reviewers for their constructive comments. This work was supported by National Natural Science Foundation of China Grant 31222033, National Basic Research Program of China (973 Program) Grant 2014CB943500, Strategic Priority Research Program of CAS Grant XDA08020105, the Hundred Talents Program of CAS to Y.J., and U.S. National Science Foundation 2010 Project Grant MCB-0929349 to E.M.M. and Y.J. The Meyerowitz Laboratory is also supported by funds from the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation (through Grant GBMF3406). Y.W. received a fellowship from the China Postdoctoral Science Foundation, and J.W. was a recipient of the Syngenta Friendly Laboratories Scholarship.
Funding AgencyGrant Number
National Natural Science Foundation of China31222033
National Basic Research Program of China (973 Program)2014CB943500
Chinese Academy of SciencesXDA08020105
Hundred Talents Program of Chinese Academy of SciencesUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Gordon and Betty Moore FoundationGBMF3406
China Postdoctoral Science FoundationUNSPECIFIED
Syngenta Friendly LaboratoriesUNSPECIFIED
Issue or Number:5
PubMed Central ID:PMC4079368
Record Number:CaltechAUTHORS:20140609-152817548
Persistent URL:
Official Citation:Wang, Y., Wang, J., Shi, B., Yu, T., Qi, J., Meyerowitz, E. M., & Jiao, Y. (2014). The Stem Cell Niche in Leaf Axils Is Established by Auxin and Cytokinin in Arabidopsis. The Plant Cell Online, 26(5), 2055-2067. doi: 10.1105/tpc.114.123083
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:46162
Deposited By: Ruth Sustaita
Deposited On:10 Jun 2014 21:47
Last Modified:10 Nov 2021 17:21

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