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Growth dynamics of geographically different arbuscular mycorrhizal fungal isolates belonging to the 'Rhizophagus clade' under monoxenic conditions

Silvani, Vanesa Anali and Fernández Bidondo, Laura and Bompadre, Maria Josefina and Colombo, Roxana Paula and Pérgola, Mariana and Bompadre, Augustín and Fracchia, Sebastián and Godeas, Alicia (2014) Growth dynamics of geographically different arbuscular mycorrhizal fungal isolates belonging to the 'Rhizophagus clade' under monoxenic conditions. Mycologia, 106 (5). pp. 963-975. ISSN 0027-5514. http://resolver.caltech.edu/CaltechAUTHORS:20140610-144141244

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Abstract

The growth dynamics of extraradical mycelium and spore formation of fourteen 'Rhizophagus' isolates from different sites in Argentina were evaluated under monoxenic conditions. A modified Gompertz model was used to characterize the development of mycelium and spores for each isolate under the same conditions. The lag time, maximal growth rate, and total quantity of both extraradical hyphae and spores were determined. Wide variability among isolates was detected and all growth parameters were significantly altered by fungal isolate. Discriminant analysis differentiated isolates primarily based on the extent of extraradical hyphae produced, yet such differences did not conclusively correspond to phylogenetic relationships among closely related isolates based on partial SSU sequences. Given that the 'Rhizophagus' isolates were grown under controlled conditions for many generations, the expression of phenotypic variability could be attributed to genetic differences that are not completely resolved by phylogenetic analysis employing the small ribosomal gene.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.mycologia.org/content/106/5/963.shortPublisherArticle
http://dx.doi.org/10.3852/13-118DOIArticle
Additional Information:© 2014 Mycological Society of America. Submitted 14 Apr 2013; accepted for publication 14 Apr 2013. In Press at Mycologia, preliminary version published on June 2, 2014 as doi:10.3852/13-118. We specially thank Dra Gisela Cuenca and her laboratory staff for helping us with Rhizophagus morphological characterization and Prof R. Larry Peterson (University of Guelph, Canada) for editing the English. We appreciate the valuable comments and suggestions of Associate Editor R. Paul Schreiner and reviewers that have substantially improved our manuscript. We also thank Dr Sergio Ghío for technical assistance and Lic. Rothen Carolina for her help in creating FIG. 3. We also gratefully acknowledge financial support from Universidad de Buenos Aires, Agencia Nacional de Promoción Científica y Tecnológica and Consejo Nacional de Investigaciones Científicas y Técnicas.
Funders:
Funding AgencyGrant Number
Universidad de Buenos AiresUNSPECIFIED
Agencia Nacional de Promoción Científica y TecnológicaUNSPECIFIED
Consejo Nacional de Investigaciones Científicas y TécnicasUNSPECIFIED
Subject Keywords:Glomeromycota, growth dynamics, in vitro, Rhizophagus intraradices, Rhizophagus irregularis, variability
Record Number:CaltechAUTHORS:20140610-144141244
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20140610-144141244
Official Citation:Growth dynamics of geographically different arbuscular mycorrhizal fungal isolates belonging to the ‘Rhizophagus clade’ under monoxenic conditions Vanesa Analía Silvani, Laura Fernández Bidondo, María Josefina Bompadre, Roxana Paula Colombo, Mariana Pérgola, Agustín Bompadre, Sebastián Fracchia, and Alicia Godeas Mycologia 2014 106:963-975; Preliminary version published online: June 2, 2014, doi:10.3852/13-118
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:46186
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:10 Jun 2014 22:19
Last Modified:15 Jan 2016 00:38

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