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Behavioral and Neural Correlates of Acute and Scheduled Hunger in C57BL/6 Mice

Gallardo, Christian M. and Hsu, Cynthia T. and Gunapala, Keith M. and Parfyonov, Maksim and Chang, Chris H. and Mistlberger, Ralph E. and Steele, Andrew D. (2014) Behavioral and Neural Correlates of Acute and Scheduled Hunger in C57BL/6 Mice. PLoS ONE, 9 (5). Art. No. e95990. ISSN 1932-6203. PMCID PMC4012955. doi:10.1371/journal.pone.0095990.

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In rodents, daily feeding schedules induce food anticipatory activity (FAA) rhythms with formal properties suggesting mediation by food-entrained circadian oscillators (FEOs). The search for the neuronal substrate of FEOs responsible for FAA is an active area of research, but studies spanning several decades have yet to identify unequivocally a brain region required for FAA. Variability of results across studies leads to questions about underlying biology versus methodology. Here we describe in C57BL/6 male mice the effects of varying the ‘dose’ of caloric restriction (0%, 60%, 80%, 110%) on the expression of FAA as measured by a video-based analysis system, and on the induction of c-Fos in brain regions that have been implicated in FAA. We determined that more severe caloric restriction (60%) leads to a faster onset of FAA with increased magnitude. Using the 60% caloric restriction, we found little evidence for unique signatures of neuronal activation in the brains of mice anticipating a daily mealtime compared to mice that were fasted acutely or fed ad-libitum–even in regions such as the dorsomedial and ventrolateral hypothalamus, nucleus accumbens, and cerebellum that have previously been implicated in FAA. These results underscore the importance of feeding schedule parameters in determining quantitative features of FAA in mice, and demonstrate dissociations between behavioral FAA and neural activity in brain areas thought to harbor FEOs or participate in their entrainment or output.

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Additional Information:© 2014 Gallardo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received: January 23, 2014; Accepted: April 1, 2014; Published: May 7, 2014. Funding for these studies was provided by the Broad Fellows Program in Brain Circuitry at Caltech, an Ellison Medical Foundation New Scholar Award to ADS, a Grants Program in Eating Disorders Research award from the Klarman Family Foundation to ADS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Conceived and designed the experiments: CMG CTH KMG ADS. Performed the experiments: CMG CTH KMG MP CHC REM ADS. Analyzed the data: CMG CTH REM ADS. Wrote the paper: CMG CTH REM ADS.
Funding AgencyGrant Number
Broad Fellows Program in Brain Circuitry, CaltechUNSPECIFIED
Ellison Medical Foundation New Scholar AwardUNSPECIFIED
Klarman Family FoundationUNSPECIFIED
Issue or Number:5
PubMed Central ID:PMC4012955
Record Number:CaltechAUTHORS:20140620-090526535
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Official Citation:Gallardo CM, Hsu CT, Gunapala KM, Parfyonov M, Chang CH, et al. (2014) Behavioral and Neural Correlates of Acute and Scheduled Hunger in C57BL/6 Mice. PLoS ONE 9(5): e95990. doi:10.1371/journal.pone.0095990
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:46388
Deposited By: Jason Perez
Deposited On:20 Jun 2014 16:39
Last Modified:10 Nov 2021 17:24

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