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Thermopressurized diluted phosphoric acid pretreatment of ligno(hemi)cellulose to make free sugars and nutraceutical oligosaccharides

Tiboni, Marcela and Grzybowski, Adelia and Baldo, Gizele Rejane and Dias, Edson Flausino, Jr. and Tanner, Robert D. and Kornfield, Julia Ann and Fontana, José Domingos (2014) Thermopressurized diluted phosphoric acid pretreatment of ligno(hemi)cellulose to make free sugars and nutraceutical oligosaccharides. Journal of Industrial Microbiology and Biotechnology, 41 (6). pp. 957-964. ISSN 1367-5435. http://resolver.caltech.edu/CaltechAUTHORS:20140620-134714828

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Abstract

Ligno(hemi)cellulosics (L(h)Cs) as sugarcane bagasse and loblolly pine sawdust are currently being used to produce biofuels such as bioethanol and biobutanol through fermentation of free sugars that are often obtained enzymatically. However, this bioconversion requires a pretreatment to solubilize the hemicellulose fractions, thus facilitating the action of the cellulolytic enzymes. Instead of the main free monosaccharides used in these current models, the modulation of thermopressurized orthophosphoric acid as a pretreatment, in the ranges of 3–12 atm and pH 1.5–2.5, can produce nondigestible oligosaccharides (NDOS) such as xylo-oligosaccharides (XOS) because heteroxylan is present in both types of hardwood and softwood hemicelluloses. A comparative thin-layer chromatographic analysis of the hydrolytic products showed the best conditions for NDOS production to be 7 atm/water, pH 2.25 and 2.50, and 8.5 atm/water for both sources. Particular hydrolysates from 7 atm (171 °C) at pHs 2.25 and 2.50 both for cane bagasse and pine sawdust, with respective oligosaccharide contents of 57 and 59 %, once mixed in a proportion of 1:1 for each plant source, were used in vitro as carbon sources for Bifidobacterium or Lactobacillus. Once both bacteria attained the stationary phase of growth, an unforeseen feature emerged: the preference of B. animalis for bagasse hydrolysates and, conversely, the preference of L. casei for pine hydrolysates. Considering the fact that nutraceutical oligosaccharides from both hemicelluloses correspond to higher value-added byproducts, the technology using a much diluted thermopressurized orthophosphoric acid pretreatment becomes an attractive choice for L(h)Cs.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1007/s10295-014-1426-3DOIArticle
http://link.springer.com/article/10.1007%2Fs10295-014-1426-3PublisherArticle
http://rdcu.be/pY7XPublisherFree ReadCube access
ORCID:
AuthorORCID
Kornfield, Julia Ann0000-0001-6746-8634
Additional Information:© 2014 Society for Industrial Microbiology and Biotechnology. Received: 12 December 2013; Accepted: 19 February 2014; Published online: 18 April 2014. The authors would like to thank the National Council for Scientific and Technological Development (CNPq), the Coordination for the Improvement of Higher Education Personnel (CAPES), and the Araucaria Foundation from SETI-PR.
Funders:
Funding AgencyGrant Number
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNSPECIFIED
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)UNSPECIFIED
SETI-PR Araucaria FoundationUNSPECIFIED
Subject Keywords:Bifidobacteria; Lactobacilli; Galactoglucomannan oligosaccharides; Xylo-oligosaccharides
Record Number:CaltechAUTHORS:20140620-134714828
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20140620-134714828
Official Citation:Tiboni, M., Grzybowski, A., Baldo, G., Dias, E., Jr., Tanner, R., Kornfield, J., & Fontana, J. (2014). Thermopressurized diluted phosphoric acid pretreatment of ligno(hemi)cellulose to make free sugars and nutraceutical oligosaccharides. Journal of Industrial Microbiology & Biotechnology, 41(6), 957-964. doi: 10.1007/s10295-014-1426-3
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:46402
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:23 Jun 2014 16:55
Last Modified:10 Mar 2017 23:33

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