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A P1-based physical map of the region from D17S776 to D17S78 containing the breast cancer susceptibility gene BRCA1

Neuhausen, S. L. and Swensen, J. and Miki, Y. and Liu, Q. and Tavtigian, S. and Shattuck-Eidens, D. and Kamb, A. and Hobbs, M. R. and Gingrich, J. and Shizuya, H. and Kim, U.-J. and Cochran, C. and Futreal, P. A. and Wiseman, R. W. and Lynch, H. T. and Tonin, P. and Narod, S. and Cannon-Albright, L. and Skolnick, M. H. and Goldgar, D. E. (1994) A P1-based physical map of the region from D17S776 to D17S78 containing the breast cancer susceptibility gene BRCA1. Human Molecular Genetics, 3 (11). pp. 1919-1926. ISSN 0964-6906. https://resolver.caltech.edu/CaltechAUTHORS:20140625-112136445

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Abstract

BRCA1, a breast and ovarian cancer susceptibility locus, has been isolated and maps to 17q21. A physical map of the BRCA1, region which extended from the proximal boundary at D17S776 to the distal boundary at D17S78 was constructed and consists of 51 sequence tagged sites (STSs) from P1 and YAC ends, nine new short-tandem repeat (STR) polymorphic markers, and eight identified genes. The contig, which spans the estimated 2.3 Mb region, contains 29 P1s, 11 YACs, two BACs, and one cosmid. Based on key recombinants in two linked families, BRCA1 was further localized to a region bounded by D17S1321 on the proximal side and D17S1325 on the distal side. Within this estimated 600 kb region, the contig was composed completely of P1s and BACs ordered by STS-content mapping and confirmed by DNA restriction fragment fingerprinting.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1093/hmg/3.11.1919DOIArticle
http://hmg.oxfordjournals.org/content/3/11/1919.abstractPublisherArticle
Additional Information:© 1994 Oxford University Press. Received August 26, 1994; accepted September 28, 1994. We thank K. Fournier from Fred Hutchinson Cancer Research Center for supplying us with the somatic cell lines. We thank M. Ashton for technical assistance in drawing the figures and L.M. Bennen for confirmation of STS-content. We are grateful to D. Smoller and K. Schriewer from Genome Systems for their careful and efficient screening of the PI library. This work was supported by grants CA-48711 and CA-55914 from the National Institutes of Health, by Myriad Genetics, the National Cancer Institute of Canada Breast Cancer Initiative, the Canadian Genetics Diseases Network, and the Cedars Cancer Institute of the Royal Victoria Hospital.
Funders:
Funding AgencyGrant Number
NIHCA-48711
NIHCA-55914
Myriad GeneticsUNSPECIFIED
National Cancer Institute of Canada Breast Cancer InitiativeUNSPECIFIED
Canadian Genetics Diseases NetworkUNSPECIFIED
Cedars Cancer Institute of the Royal Victoria HospitalUNSPECIFIED
Issue or Number:11
Record Number:CaltechAUTHORS:20140625-112136445
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20140625-112136445
Official Citation:Neuhausen, S. L., Swensen, J., Miki, Y., Llu, Q., Tavtiglan, S., Shattuck-Eidens, D., . . . Goldgar, D. E. (1994). A P1-based physical map of the region from D17S776 to D17S78 containing the breast cancer susceptibility gene BRCA1. Human Molecular Genetics, 3(11), 1919-1926. doi: 10.1093/hmg/3.11.1919
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:46503
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:26 Jun 2014 18:28
Last Modified:03 Oct 2019 06:44

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