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How Embryonic Cartilage Grows: Insights Gained from Quantitative Live Imaging

Trivedi, Vikas and Li, Yuwei and Truong, Thai V. and Koos, David and Cheng-Ming, Chuong and Moats, Rex and Fraser, Scott E. (2014) How Embryonic Cartilage Grows: Insights Gained from Quantitative Live Imaging. Biophysical Journal, 106 (2). 575A. ISSN 0006-3495. https://resolver.caltech.edu/CaltechAUTHORS:20140710-091017762

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Abstract

One central question in development biology is how individual cell behaviors contribute to tissue morphogenesis. Growth plate cartilage contains morphologically distinct populations of chondrocytes in different zones during much of its growth phase, indicating a link between regulated cell behaviors and tissue elongation. To gain insights into this process, we develop a robust avian embryonic metacarpal culture system, and employ time-lapsed live 2-photon laser scanning microscopy to observe the cartilage growth. Quantitative analysis of the cellular displacements during the tissue growth reveals that cells in the proliferative and prehypertrophic zones, though morphologically distinct, display similar displacement trajectories, contributing in a linearly additive fashion to the unidirectional tissue growth. Our analysis rules out cell division and convergence-extension as the driving mechanisms for tissue elongation; rather, anisotropic matrix deposition and cell volume enlargement are responsible for sculpting the directional tissue growth.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.bpj.2013.11.3188DOIArticle
http://www.sciencedirect.com/science/article/pii/S0006349513044470PublisherArticle
http://www.biophysics.org/2014meeting/Main/tabid/4177/Default.aspxOrganizationConference Website
ORCID:
AuthorORCID
Trivedi, Vikas0000-0003-0953-0553
Li, Yuwei0000-0001-7753-4869
Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 2014 Biophysical Society. Published by Elsevier Inc.
Issue or Number:2
Record Number:CaltechAUTHORS:20140710-091017762
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20140710-091017762
Official Citation:Vikas Trivedi, Yuwei Li, Thai V. Truong, David Koos, Chuong Cheng-Ming, Rex Moats, Scott E. Fraser, How Embryonic Cartilage Grows: Insights Gained from Quantitative Live Imaging, Biophysical Journal, Volume 106, Issue 2, Supplement 1, 28 January 2014, Page 575a, ISSN 0006-3495, http://dx.doi.org/10.1016/j.bpj.2013.11.3188. (http://www.sciencedirect.com/science/article/pii/S0006349513044470)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:47124
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:10 Jul 2014 16:15
Last Modified:03 Oct 2019 06:50

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