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Sensing and Stiffening of Tubular Membranes by the Syndapin 1 FBAR

Ramesh, Pradeep and Baroji, Younes F. and Reihani, Nader S. and Stamou, Dimitrios and Oddershede, Lene B. and Bendix, Poul M. (2014) Sensing and Stiffening of Tubular Membranes by the Syndapin 1 FBAR. Biophysical Journal, 106 (2). 714A. ISSN 0006-3495.

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Bin-Amphiphysin-Rvs (BAR) domains are essential components of the cellular machinery responsible for membrane deformation and were found to be sensitive sensors of membrane curvature. One common feature of BAR domains is a curved shape which correlates with high membrane curvatures often found in cells. We focused on characterizing the FBAR domain of Syndapin-1 in vitro, given its medical relevance to neurological diseases, using tubular membranes pulled out of Giant Unilamellar Vesicles (GUVs). Specifically, GUVs were synthesized using either porcine brain lipid extracts or the more conventional, binary lipid mixtures to systematically test how lipid composition affects curvature sensing activity. Using optical trapping coupled with force-spectroscopy and confocal microscopy, we discovered an inverse relationship between the curvature sensing activity of the FBAR domain and its equilibrium concentration in solution. At high bulk concentrations of protein, we explicitly measured an increase in the tube's persistence length, which can be understood as mechanical stiffening of the tube. Lastly, we used force spectroscopy to accurately test the effect of the protein on membrane relaxation dynamics in real time.

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Additional Information:© 2014 Biophysical Society. Published by Elsevier Inc.
Record Number:CaltechAUTHORS:20140710-103526444
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Official Citation:Pradeep Ramesh, Younes F. Baroji, Nader S. Reihani, Dimitrios Stamou, Lene B. Oddershede, Poul M. Bendix, Sensing and Stiffening of Tubular Membranes by the Syndapin 1 FBAR, Biophysical Journal, Volume 106, Issue 2, Supplement 1, 28 January 2014, Page 714a, ISSN 0006-3495, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:47138
Deposited By: Tony Diaz
Deposited On:10 Jul 2014 19:31
Last Modified:10 Jul 2014 19:31

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